Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1158 | 3' | -54.5 | NC_001132.2 | + | 6691 | 0.66 | 0.944783 |
Target: 5'- ----cGGAGGaCGGACGAAcuCGGcGGAGa -3' miRNA: 3'- uucuaUCUCC-GCCUGCUU--GCC-CCUUg -5' |
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1158 | 3' | -54.5 | NC_001132.2 | + | 126181 | 0.7 | 0.80758 |
Target: 5'- uGGGGUAGGGGCGGuCGu-CGGuGGcGCg -3' miRNA: 3'- -UUCUAUCUCCGCCuGCuuGCC-CCuUG- -5' |
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1158 | 3' | -54.5 | NC_001132.2 | + | 10722 | 1.08 | 0.004538 |
Target: 5'- cAAGAUAGAGGCGGACGAACGGGGAACg -3' miRNA: 3'- -UUCUAUCUCCGCCUGCUUGCCCCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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