Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11581 | 5' | -56.7 | NC_003085.1 | + | 19603 | 0.66 | 0.550374 |
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Target: 5'- gUCggGCGCCgucCGCug-GGCUGCUcGAa -3' miRNA: 3'- gAGa-CGCGGa--GCGuaaCCGACGA-CUc -5' |
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| 11581 | 5' | -56.7 | NC_003085.1 | + | 30219 | 0.66 | 0.53951 |
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Target: 5'- --gUGaCGUCcUGCGUgGGCUGCUGGGg -3' miRNA: 3'- gagAC-GCGGaGCGUAaCCGACGACUC- -5' |
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| 11581 | 5' | -56.7 | NC_003085.1 | + | 20635 | 0.67 | 0.518008 |
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Target: 5'- aUCUGCGUCUgGaUGUUGG-UGCUGAa -3' miRNA: 3'- gAGACGCGGAgC-GUAACCgACGACUc -5' |
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| 11581 | 5' | -56.7 | NC_003085.1 | + | 24903 | 0.68 | 0.465886 |
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Target: 5'- aUCUGCGCCUCGgCGUccUGGUUGa---- -3' miRNA: 3'- gAGACGCGGAGC-GUA--ACCGACgacuc -5' |
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| 11581 | 5' | -56.7 | NC_003085.1 | + | 23069 | 0.68 | 0.455786 |
|
Target: 5'- -aCUGCGgCgcgCGCAccucUGGCUGCUuGGGg -3' miRNA: 3'- gaGACGCgGa--GCGUa---ACCGACGA-CUC- -5' |
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| 11581 | 5' | -56.7 | NC_003085.1 | + | 17929 | 0.7 | 0.353277 |
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Target: 5'- -cCUGCGCCcagCGCGgcgcucugGGCucUGCUGGGg -3' miRNA: 3'- gaGACGCGGa--GCGUaa------CCG--ACGACUC- -5' |
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| 11581 | 5' | -56.7 | NC_003085.1 | + | 30676 | 1.09 | 0.000489 |
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Target: 5'- gCUCUGCGCCUCGCAUUGGCUGCUGAGg -3' miRNA: 3'- -GAGACGCGGAGCGUAACCGACGACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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