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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11584 | 5' | -52.1 | NC_003085.1 | + | 10843 | 0.66 | 0.824202 |
Target: 5'- gGGAGUGGGAUGCgagC-UCCGg----- -3' miRNA: 3'- -CCUCACCCUACGga-GuAGGCaacaac -5' |
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11584 | 5' | -52.1 | NC_003085.1 | + | 5826 | 0.69 | 0.688077 |
Target: 5'- -aAGgGGGAUGCgCUCAUCCGUa---- -3' miRNA: 3'- ccUCaCCCUACG-GAGUAGGCAacaac -5' |
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11584 | 5' | -52.1 | NC_003085.1 | + | 31576 | 1.1 | 0.001506 |
Target: 5'- uGGAGUGGGAUGCCUCAUCCGUUGUUGu -3' miRNA: 3'- -CCUCACCCUACGGAGUAGGCAACAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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