Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11585 | 3' | -53.1 | NC_003085.1 | + | 18456 | 0.66 | 0.847634 |
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Target: 5'- ---aCGUCCUGGAGG--AGCGcGUGGa -3' miRNA: 3'- ggaaGCAGGGCCUUUcaUCGCaCGCC- -5' |
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| 11585 | 3' | -53.1 | NC_003085.1 | + | 43871 | 0.66 | 0.829975 |
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Target: 5'- aCCUgCaUCCgGGcaaguAGUGGCGUGcCGGg -3' miRNA: 3'- -GGAaGcAGGgCCuu---UCAUCGCAC-GCC- -5' |
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| 11585 | 3' | -53.1 | NC_003085.1 | + | 18992 | 0.66 | 0.820827 |
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Target: 5'- gCCUUCGUCCggugcaucguCGGGcccguuGGUAGCG-GCa- -3' miRNA: 3'- -GGAAGCAGG----------GCCUu-----UCAUCGCaCGcc -5' |
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| 11585 | 3' | -53.1 | NC_003085.1 | + | 35911 | 0.67 | 0.792225 |
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Target: 5'- gCCUugcUCGcCUCGGugugaacccgucGGGGUAGCGcuUGCGGa -3' miRNA: 3'- -GGA---AGCaGGGCC------------UUUCAUCGC--ACGCC- -5' |
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| 11585 | 3' | -53.1 | NC_003085.1 | + | 45619 | 0.69 | 0.654759 |
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Target: 5'- gCCgUgGUCCUGGAug--GGCaGUGCGGg -3' miRNA: 3'- -GGaAgCAGGGCCUuucaUCG-CACGCC- -5' |
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| 11585 | 3' | -53.1 | NC_003085.1 | + | 31761 | 1.13 | 0.001034 |
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Target: 5'- aCCUUCGUCCCGGAAAGUAGCGUGCGGu -3' miRNA: 3'- -GGAAGCAGGGCCUUUCAUCGCACGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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