Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11586 | 3' | -58 | NC_003085.1 | + | 45121 | 0.71 | 0.288003 |
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Target: 5'- --aGCCG-GCGGAG-GAGGCuGGCGAc -3' miRNA: 3'- cuaCGGCaCGCCUUgCUCCGcCUGCU- -5' |
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| 11586 | 3' | -58 | NC_003085.1 | + | 9098 | 0.71 | 0.280937 |
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Target: 5'- cGGUGUgGUGUGGAagaGCcGGGCGGACu- -3' miRNA: 3'- -CUACGgCACGCCU---UGcUCCGCCUGcu -5' |
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| 11586 | 3' | -58 | NC_003085.1 | + | 44071 | 0.71 | 0.280937 |
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Target: 5'- --gGCCcgaGUGCGGGAguugGAGGCGGACa- -3' miRNA: 3'- cuaCGG---CACGCCUUg---CUCCGCCUGcu -5' |
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| 11586 | 3' | -58 | NC_003085.1 | + | 42060 | 0.72 | 0.260566 |
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Target: 5'- --aGuCCGcGCGGAGCGGGGUGaaGACGAu -3' miRNA: 3'- cuaC-GGCaCGCCUUGCUCCGC--CUGCU- -5' |
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| 11586 | 3' | -58 | NC_003085.1 | + | 15208 | 0.72 | 0.254048 |
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Target: 5'- --cGCCGcgccGCGGAaguggGCGAGGUGGACu- -3' miRNA: 3'- cuaCGGCa---CGCCU-----UGCUCCGCCUGcu -5' |
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| 11586 | 3' | -58 | NC_003085.1 | + | 17885 | 0.75 | 0.158305 |
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Target: 5'- -cUGCCGcacagGCGGcacCGGGGCGGGCGGu -3' miRNA: 3'- cuACGGCa----CGCCuu-GCUCCGCCUGCU- -5' |
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| 11586 | 3' | -58 | NC_003085.1 | + | 31895 | 1.09 | 0.000515 |
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Target: 5'- cGAUGCCGUGCGGAACGAGGCGGACGAg -3' miRNA: 3'- -CUACGGCACGCCUUGCUCCGCCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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