Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11589 | 5' | -54.9 | NC_003085.1 | + | 19345 | 0.66 | 0.674924 |
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Target: 5'- -cGAgGCCCGGCAGgugccuaaGGccaUCCUCGa -3' miRNA: 3'- aaCU-CGGGCCGUCauaag---UC---AGGAGC- -5' |
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| 11589 | 5' | -54.9 | NC_003085.1 | + | 44017 | 0.66 | 0.670442 |
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Target: 5'- -cGGGCCuCGGCGGUGgagagacgggaagCGGUCCg-- -3' miRNA: 3'- aaCUCGG-GCCGUCAUaa-----------GUCAGGagc -5' |
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| 11589 | 5' | -54.9 | NC_003085.1 | + | 45549 | 0.66 | 0.65471 |
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Target: 5'- -cGAGCCUGGC-GUG-UCAacUCCUCGc -3' miRNA: 3'- aaCUCGGGCCGuCAUaAGUc-AGGAGC- -5' |
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| 11589 | 5' | -54.9 | NC_003085.1 | + | 19012 | 0.68 | 0.564868 |
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Target: 5'- -cGGGCCCGuuGGUAgcggcaAGUCCUCu -3' miRNA: 3'- aaCUCGGGCcgUCAUaag---UCAGGAGc -5' |
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| 11589 | 5' | -54.9 | NC_003085.1 | + | 40451 | 0.68 | 0.564868 |
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Target: 5'- -aGAGCCgGGCGGacuugaggAggUAGUCCUCu -3' miRNA: 3'- aaCUCGGgCCGUCa-------UaaGUCAGGAGc -5' |
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| 11589 | 5' | -54.9 | NC_003085.1 | + | 5229 | 0.68 | 0.531863 |
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Target: 5'- --cGGCCCGGCAGgaagCGG-CCUCc -3' miRNA: 3'- aacUCGGGCCGUCauaaGUCaGGAGc -5' |
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| 11589 | 5' | -54.9 | NC_003085.1 | + | 10641 | 0.71 | 0.402983 |
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Target: 5'- -gGGGCgCCGGCAGaggcagccaaAGUCCUCGg -3' miRNA: 3'- aaCUCG-GGCCGUCauaag-----UCAGGAGC- -5' |
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| 11589 | 5' | -54.9 | NC_003085.1 | + | 33247 | 1.06 | 0.001372 |
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Target: 5'- gUUGAGCCCGGCAGUAUUCAGUCCUCGc -3' miRNA: 3'- -AACUCGGGCCGUCAUAAGUCAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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