Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11592 | 5' | -59.8 | NC_003085.1 | + | 41025 | 0.66 | 0.462874 |
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Target: 5'- uGGGcGUcgcuggcguccuccUGGGcGgCUUGGCGGG-CUGCu -3' miRNA: 3'- -CCCuCA--------------ACCC-CgGGAUCGCCCaGACG- -5' |
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| 11592 | 5' | -59.8 | NC_003085.1 | + | 3917 | 0.66 | 0.436962 |
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Target: 5'- aGGAGUUGacacgccaGGCUCgcgucGCGGGcCUGCa -3' miRNA: 3'- cCCUCAACc-------CCGGGau---CGCCCaGACG- -5' |
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| 11592 | 5' | -59.8 | NC_003085.1 | + | 26084 | 0.67 | 0.382516 |
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Target: 5'- aGGAGUugguUGGGGUCgaAGUgcuGGGgCUGCg -3' miRNA: 3'- cCCUCA----ACCCCGGgaUCG---CCCaGACG- -5' |
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| 11592 | 5' | -59.8 | NC_003085.1 | + | 11439 | 0.68 | 0.365397 |
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Target: 5'- gGGGAagcggucgccGUUGGGGCCgacGCGGuGUgaGCg -3' miRNA: 3'- -CCCU----------CAACCCCGGgauCGCC-CAgaCG- -5' |
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| 11592 | 5' | -59.8 | NC_003085.1 | + | 15073 | 0.68 | 0.357039 |
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Target: 5'- uGGGAGUU--GGCCCUGGaggaGGGaCgGCg -3' miRNA: 3'- -CCCUCAAccCCGGGAUCg---CCCaGaCG- -5' |
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| 11592 | 5' | -59.8 | NC_003085.1 | + | 26650 | 0.68 | 0.352088 |
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Target: 5'- cGGAGUcGGGGCCgUcgccuccugGGCGGGagcggccguugccgCUGCc -3' miRNA: 3'- cCCUCAaCCCCGGgA---------UCGCCCa-------------GACG- -5' |
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| 11592 | 5' | -59.8 | NC_003085.1 | + | 16703 | 0.68 | 0.324962 |
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Target: 5'- cGGGGGcUUGGGGCUUgcGaCGGGUUUcaaGCc -3' miRNA: 3'- -CCCUC-AACCCCGGGauC-GCCCAGA---CG- -5' |
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| 11592 | 5' | -59.8 | NC_003085.1 | + | 35064 | 1.12 | 0.000204 |
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Target: 5'- uGGGAGUUGGGGCCCUAGCGGGUCUGCc -3' miRNA: 3'- -CCCUCAACCCCGGGAUCGCCCAGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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