Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11597 | 3' | -55.3 | NC_003085.1 | + | 41030 | 0.66 | 0.687689 |
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Target: 5'- -aCGGCUGCUGCuGGCcGAGCaccugaaacugCCAGCg -3' miRNA: 3'- caGCUGACGAUG-CUGcCUUGg----------GGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 41143 | 0.66 | 0.713699 |
|
Target: 5'- -cCGuACUGCUggccggaugccuccaACGGCuaccugggcaaGGGGCUCCAGCg -3' miRNA: 3'- caGC-UGACGA---------------UGCUG-----------CCUUGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 43363 | 0.71 | 0.37674 |
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Target: 5'- gGUCGGCaUGCagGCGACGGAGgacuCCCUggGGCc -3' miRNA: 3'- -CAGCUG-ACGa-UGCUGCCUU----GGGG--UCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 47487 | 0.72 | 0.341657 |
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Target: 5'- --gGGCUGCUcaugcacGCGGCGGAACCUguCGGUg -3' miRNA: 3'- cagCUGACGA-------UGCUGCCUUGGG--GUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 47713 | 0.73 | 0.309764 |
|
Target: 5'- cGUCGACUGCUGCGccugcugacgggcGCGGuGCgCgUAGCg -3' miRNA: 3'- -CAGCUGACGAUGC-------------UGCCuUG-GgGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 48710 | 0.69 | 0.534374 |
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Target: 5'- ---aACUGCgGCGAC-GAACUCCGGCa -3' miRNA: 3'- cagcUGACGaUGCUGcCUUGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 49110 | 0.7 | 0.471843 |
|
Target: 5'- cUCG--UGCU--GACGGGGCCCCGGUg -3' miRNA: 3'- cAGCugACGAugCUGCCUUGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 49290 | 0.69 | 0.513162 |
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Target: 5'- -gUGGCUGCUGCGucgcguCGGcgUCCCGGg -3' miRNA: 3'- caGCUGACGAUGCu-----GCCuuGGGGUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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