Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11597 | 3' | -55.3 | NC_003085.1 | + | 9755 | 0.68 | 0.576555 |
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Target: 5'- cUUGcGCUGCgccucaACGGCGGAgagacgaGCCuCCAGCg -3' miRNA: 3'- cAGC-UGACGa-----UGCUGCCU-------UGG-GGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 9049 | 0.74 | 0.280901 |
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Target: 5'- -gCGGCUGCacggGCGGCaGGuguggugguucGGCCCCAGCa -3' miRNA: 3'- caGCUGACGa---UGCUG-CC-----------UUGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 5822 | 0.67 | 0.654759 |
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Target: 5'- --gGGCUGCUGgaGGCGGAuggccuCCCguGCc -3' miRNA: 3'- cagCUGACGAUg-CUGCCUu-----GGGguCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 4114 | 0.66 | 0.709392 |
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Target: 5'- aGUUGGgcGCcGCGcUGGGACUCCAGCg -3' miRNA: 3'- -CAGCUgaCGaUGCuGCCUUGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 2889 | 0.7 | 0.45579 |
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Target: 5'- aUCG-CUGCgUAUGucgggaaggaccucuACGGGGCCCCAGUc -3' miRNA: 3'- cAGCuGACG-AUGC---------------UGCCUUGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 2787 | 0.66 | 0.687689 |
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Target: 5'- -gCGGCgGCgcuccagGCGGCGGcgaagcGCCUCGGCa -3' miRNA: 3'- caGCUGaCGa------UGCUGCCu-----UGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 1345 | 0.66 | 0.698573 |
|
Target: 5'- --aGACUGCUgGCGACGuuGCCgCCAaGUa -3' miRNA: 3'- cagCUGACGA-UGCUGCcuUGG-GGU-CG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 601 | 0.66 | 0.717993 |
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Target: 5'- -gCGACUGCaagcccgucgcggccUACGGCGGGuugugcugcgccaagGCgCUCGGCg -3' miRNA: 3'- caGCUGACG---------------AUGCUGCCU---------------UG-GGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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