Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11597 | 3' | -55.3 | NC_003085.1 | + | 32259 | 0.67 | 0.607296 |
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Target: 5'- -gCGGC-GCUgaccaacucuccaaGCGcGCGGAACuCCCGGCa -3' miRNA: 3'- caGCUGaCGA--------------UGC-UGCCUUG-GGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 23356 | 0.67 | 0.599587 |
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Target: 5'- -cCGGCcGCcgaGGCGcAGCCCCAGCa -3' miRNA: 3'- caGCUGaCGaugCUGCcUUGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 37139 | 0.68 | 0.566741 |
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Target: 5'- --gGGCacgGCgACGGCGGGcACCCUGGCg -3' miRNA: 3'- cagCUGa--CGaUGCUGCCU-UGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 21157 | 0.68 | 0.545097 |
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Target: 5'- gGUgGACUGgUACGugGaGGGCUaCAGCg -3' miRNA: 3'- -CAgCUGACgAUGCugC-CUUGGgGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 11801 | 0.68 | 0.545097 |
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Target: 5'- uGUCGGCUGCcccauCGcCGGGuGCCCCGacGCu -3' miRNA: 3'- -CAGCUGACGau---GCuGCCU-UGGGGU--CG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 49290 | 0.69 | 0.513162 |
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Target: 5'- -gUGGCUGCUGCGucgcguCGGcgUCCCGGg -3' miRNA: 3'- caGCUGACGAUGCu-----GCCuuGGGGUCg -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 49110 | 0.7 | 0.471843 |
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Target: 5'- cUCG--UGCU--GACGGGGCCCCGGUg -3' miRNA: 3'- cAGCugACGAugCUGCCUUGGGGUCG- -5' |
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| 11597 | 3' | -55.3 | NC_003085.1 | + | 37718 | 1.13 | 0.000518 |
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Target: 5'- cGUCGACUGCUACGACGGAACCCCAGCg -3' miRNA: 3'- -CAGCUGACGAUGCUGCCUUGGGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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