Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
116 | 3' | -53.3 | AC_000006.1 | + | 25436 | 0.66 | 0.719662 |
Target: 5'- uCAUCGCCaccaUgAGcAAGGAGAuucCCACCCc -3' miRNA: 3'- -GUAGUGGg---AgUC-UUCUUCU---GGUGGGc -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 5176 | 0.66 | 0.719662 |
Target: 5'- --cCACCCcuUCAccgugcGGAGACCACCCu -3' miRNA: 3'- guaGUGGG--AGUcuu---CUUCUGGUGGGc -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 7075 | 0.66 | 0.719662 |
Target: 5'- --aCGCCCUCcccguGGAGAAGGgCugCUGu -3' miRNA: 3'- guaGUGGGAGu----CUUCUUCUgGugGGC- -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 27373 | 0.66 | 0.708445 |
Target: 5'- uGUUAUCCUUGGuAGggGGCCACaagCCa -3' miRNA: 3'- gUAGUGGGAGUCuUCuuCUGGUG---GGc -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 130 | 0.67 | 0.651388 |
Target: 5'- gGUCGCCg-CGGAGGcguGGCCuaGCCCGg -3' miRNA: 3'- gUAGUGGgaGUCUUCuu-CUGG--UGGGC- -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 15378 | 0.67 | 0.61682 |
Target: 5'- gAUCGCCCaggcgccauUCGGAGcacgcccgccauGggGcGCCGCCCGa -3' miRNA: 3'- gUAGUGGG---------AGUCUU------------CuuC-UGGUGGGC- -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 2860 | 0.68 | 0.593828 |
Target: 5'- gCAUUGCCCUCGGuAGA-GACU-CCCa -3' miRNA: 3'- -GUAGUGGGAGUCuUCUuCUGGuGGGc -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 24134 | 0.68 | 0.581239 |
Target: 5'- gCGUCugCCUCAGcgcucggGGGAGGACgAUCuCGg -3' miRNA: 3'- -GUAGugGGAGUC-------UUCUUCUGgUGG-GC- -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 18184 | 0.68 | 0.548349 |
Target: 5'- --gCGCCCUUGGggGcAAGGCUguuguaagccguGCCCGa -3' miRNA: 3'- guaGUGGGAGUCuuC-UUCUGG------------UGGGC- -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 10770 | 0.69 | 0.510573 |
Target: 5'- --aCACCCccgcgugcagcugCAGAAGGAcGugCGCCCGg -3' miRNA: 3'- guaGUGGGa------------GUCUUCUU-CugGUGGGC- -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 12613 | 0.71 | 0.391511 |
Target: 5'- --aCACCgaCAGucuGAGGGCCACCCu -3' miRNA: 3'- guaGUGGgaGUCuu-CUUCUGGUGGGc -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 15719 | 0.74 | 0.25295 |
Target: 5'- uCGUCGCCC-CGG-AGAuuuacGGACCACCCc -3' miRNA: 3'- -GUAGUGGGaGUCuUCU-----UCUGGUGGGc -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 22561 | 0.75 | 0.239598 |
Target: 5'- aCGUC-CUCUCGGAAGAcccGGAgcCCACCCGc -3' miRNA: 3'- -GUAGuGGGAGUCUUCU---UCU--GGUGGGC- -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 4774 | 0.76 | 0.20305 |
Target: 5'- cCGUCGuCCCggaGGAGGggGGCCACCuCGu -3' miRNA: 3'- -GUAGU-GGGag-UCUUCuuCUGGUGG-GC- -5' |
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116 | 3' | -53.3 | AC_000006.1 | + | 1222 | 1.08 | 0.001035 |
Target: 5'- uCAUCACCCUCAGAAGAAGACCACCCGu -3' miRNA: 3'- -GUAGUGGGAGUCUUCUUCUGGUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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