Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
116 | 5' | -55.2 | AC_000006.1 | + | 16247 | 0.66 | 0.57145 |
Target: 5'- gGUCaAGGU---GCGCCCCaucaaGCAGGUGg -3' miRNA: 3'- -CAG-UCCGcuuUGCGGGGa----CGUUCACg -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 18058 | 0.66 | 0.57145 |
Target: 5'- -cCAGaGUGAAGCGCgCCUUGUAcgaguacuGGUGUc -3' miRNA: 3'- caGUC-CGCUUUGCG-GGGACGU--------UCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 16916 | 0.66 | 0.537745 |
Target: 5'- -gCAGGCGucgcAGCGCCgUUGau-GUGCg -3' miRNA: 3'- caGUCCGCu---UUGCGGgGACguuCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 21756 | 0.67 | 0.531082 |
Target: 5'- -gCAGcGCGGcgcggggaugaugcuGAUGCCCgugcaCUGCGAGUGUa -3' miRNA: 3'- caGUC-CGCU---------------UUGCGGG-----GACGUUCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 9791 | 0.67 | 0.526657 |
Target: 5'- ---cGGUGguAgGCCCCUGUguugaugguguAAGUGCa -3' miRNA: 3'- caguCCGCuuUgCGGGGACG-----------UUCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 5124 | 0.67 | 0.493944 |
Target: 5'- cGUCAcGGUGAAgggguGgGCCCCuggcugggcgcuUGCAagGGUGCg -3' miRNA: 3'- -CAGU-CCGCUU-----UgCGGGG------------ACGU--UCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 16321 | 0.67 | 0.493944 |
Target: 5'- -cCAGGCccGGCGCCaCCUGCuugauGGgGCg -3' miRNA: 3'- caGUCCGcuUUGCGG-GGACGu----UCaCG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 29717 | 0.67 | 0.493944 |
Target: 5'- aGUCGGGCGAGGCGUgCgaaaCUGCcgcaAAG-GCa -3' miRNA: 3'- -CAGUCCGCUUUGCGgG----GACG----UUCaCG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 4713 | 0.67 | 0.483245 |
Target: 5'- gGUCGGGcCGuAGAUGaCCCCgaugacggguUGCAGGUGg -3' miRNA: 3'- -CAGUCC-GC-UUUGC-GGGG----------ACGUUCACg -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 23287 | 0.67 | 0.472658 |
Target: 5'- -cCAGaGCGAggAGCGCgUCggcGCGGGUGCg -3' miRNA: 3'- caGUC-CGCU--UUGCGgGGa--CGUUCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 21002 | 0.68 | 0.430543 |
Target: 5'- -gCAGGCGGcggagucGgGCCCCUGCAcggucugGGUGg -3' miRNA: 3'- caGUCCGCUu------UgCGGGGACGU-------UCACg -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 30564 | 0.68 | 0.420608 |
Target: 5'- --uGGGUGAugacuaugggguuGACGCCCaccaUGCAGGUGa -3' miRNA: 3'- cagUCCGCU-------------UUGCGGGg---ACGUUCACg -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 30533 | 0.69 | 0.40116 |
Target: 5'- aGUCGGGCGAgaccAACggcugcauccacuGCUCCUGCGaaagccccgAGUGUa -3' miRNA: 3'- -CAGUCCGCU----UUG-------------CGGGGACGU---------UCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 25082 | 0.69 | 0.392598 |
Target: 5'- -gCGGGCGAAACGauguuCCCCccgcagucuUGCAAGcagGCg -3' miRNA: 3'- caGUCCGCUUUGC-----GGGG---------ACGUUCa--CG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 3208 | 0.7 | 0.347245 |
Target: 5'- aGUCcaGGGU---GCGCgCUUGCGAGUGCg -3' miRNA: 3'- -CAG--UCCGcuuUGCGgGGACGUUCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 17874 | 0.7 | 0.338636 |
Target: 5'- cUCAGGUacuccGAGGCGUCCUgcccgGCGAuGUGCa -3' miRNA: 3'- cAGUCCG-----CUUUGCGGGGa----CGUU-CACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 14230 | 0.72 | 0.261136 |
Target: 5'- -cCGGGUGAAAgGCCUC-GUAGGUGUa -3' miRNA: 3'- caGUCCGCUUUgCGGGGaCGUUCACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 9670 | 0.72 | 0.240884 |
Target: 5'- -gCGGGUGAccccGACGCCCCUGagcGGcUGCa -3' miRNA: 3'- caGUCCGCU----UUGCGGGGACgu-UC-ACG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 8951 | 0.73 | 0.215937 |
Target: 5'- aUCA-GCGAGAUGCCCCUGCGcc-GCu -3' miRNA: 3'- cAGUcCGCUUUGCGGGGACGUucaCG- -5' |
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116 | 5' | -55.2 | AC_000006.1 | + | 15461 | 0.74 | 0.18269 |
Target: 5'- cUCGGGCG--GCGCCCCaugGCGGgcGUGCu -3' miRNA: 3'- cAGUCCGCuuUGCGGGGa--CGUU--CACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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