Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1160 | 5' | -45.8 | NC_001132.2 | + | 27278 | 1.1 | 0.02837 |
Target: 5'- aACACGGCGUCGUGUAUAAAACGUAUCc -3' miRNA: 3'- -UGUGCCGCAGCACAUAUUUUGCAUAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 159699 | 0.74 | 0.981309 |
Target: 5'- --cCGGCgGUCGUcGUAcAAGACGUGUCg -3' miRNA: 3'- uguGCCG-CAGCA-CAUaUUUUGCAUAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 49217 | 0.73 | 0.988685 |
Target: 5'- gACGCGGCGUCGuUGUuUAAAuACG-AUCc -3' miRNA: 3'- -UGUGCCGCAGC-ACAuAUUU-UGCaUAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 9712 | 0.72 | 0.995279 |
Target: 5'- uUACGGCGgUGUGUAUGAacGACGU-UCu -3' miRNA: 3'- uGUGCCGCaGCACAUAUU--UUGCAuAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 148440 | 0.7 | 0.997997 |
Target: 5'- gACGCGGUcguGUCGUGUucggugcAUGUAUCg -3' miRNA: 3'- -UGUGCCG---CAGCACAuauuu--UGCAUAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 81488 | 0.7 | 0.99863 |
Target: 5'- uACACGGCGUCucGUAUGuucuuACGUacGUCu -3' miRNA: 3'- -UGUGCCGCAGcaCAUAUuu---UGCA--UAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 6778 | 0.69 | 0.999404 |
Target: 5'- cCGgGGCG-CGUGg---GAACGUGUCg -3' miRNA: 3'- uGUgCCGCaGCACauauUUUGCAUAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 159606 | 0.69 | 0.999523 |
Target: 5'- gAUACGGCGUCGg----AAuACGUGUa -3' miRNA: 3'- -UGUGCCGCAGCacauaUUuUGCAUAg -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 25773 | 0.68 | 0.999818 |
Target: 5'- aACGCGGaCGaCGcgGUGgauuuugAAAACGUAUCg -3' miRNA: 3'- -UGUGCC-GCaGCa-CAUa------UUUUGCAUAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 127012 | 0.67 | 0.999953 |
Target: 5'- -gACGGgaccgacagucuaCGUCGUGaUGUAAAcCGUGUCu -3' miRNA: 3'- ugUGCC-------------GCAGCAC-AUAUUUuGCAUAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 22413 | 0.67 | 0.999954 |
Target: 5'- gACACGGa--CGUGUAUuauuuuccaAAAACGUGUa -3' miRNA: 3'- -UGUGCCgcaGCACAUA---------UUUUGCAUAg -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 104577 | 0.66 | 0.999982 |
Target: 5'- uACACGuCGUa-UGUAcGGGACGUAUCa -3' miRNA: 3'- -UGUGCcGCAgcACAUaUUUUGCAUAG- -5' |
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1160 | 5' | -45.8 | NC_001132.2 | + | 6531 | 0.66 | 0.999991 |
Target: 5'- uAUACGGguaugUGUCGUGUAcgccuCGUAUCc -3' miRNA: 3'- -UGUGCC-----GCAGCACAUauuuuGCAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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