miRNA: 3'- gaCUUCCGCUGaCCGUG----UCa----GUGGUG- -5'
miRNA: 3'- -GACUUCCGCUG-------------ACCGUGuCAGu--GGUG- -5'
miRNA: 3'- -GACUUCCGCUGaCCG-----------UGUCA-------GUGGUG- -5'
miRNA: 3'- gaCUUCCGCUGA--CC-GUGUCa---GUG-GUG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11606 | 3' | -55.2 | NC_003085.1 | + | 39024 | 0.66 | 0.658967 |
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Target: 5'- -aGGAGGCGAC-GGCAUgcguGGgcgagCGCUGCg -3' miRNA: 3'- gaCUUCCGCUGaCCGUG----UCa----GUGGUG- -5' |
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| 11606 | 3' | -55.2 | NC_003085.1 | + | 17914 | 0.66 | 0.669019 |
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Target: 5'- uCUGGAGGUGGCaugucgagcccugUGGUGCcGUCuucCCACu -3' miRNA: 3'- -GACUUCCGCUG-------------ACCGUGuCAGu--GGUG- -5' |
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| 11606 | 3' | -55.2 | NC_003085.1 | + | 7484 | 0.66 | 0.669019 |
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Target: 5'- gCUGGAGGUGACgGGCguccgcgucgaGCGGUugcaggaCAUCACc -3' miRNA: 3'- -GACUUCCGCUGaCCG-----------UGUCA-------GUGGUG- -5' |
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| 11606 | 3' | -55.2 | NC_003085.1 | + | 7330 | 0.66 | 0.681265 |
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Target: 5'- -cGGAGGCGGCUgaGGaCGCGGgcuaCACgCGCc -3' miRNA: 3'- gaCUUCCGCUGA--CC-GUGUCa---GUG-GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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