Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11606 | 3' | -55.2 | NC_003085.1 | + | 5322 | 0.72 | 0.325288 |
Target: 5'- cCUGAAGGCGACcgagaaGCGC-GUgGCCGCg -3' miRNA: 3'- -GACUUCCGCUGac----CGUGuCAgUGGUG- -5' |
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11606 | 3' | -55.2 | NC_003085.1 | + | 3993 | 0.67 | 0.625354 |
Target: 5'- -cGAGcGCGACacGGCACAGUgGCC-Ca -3' miRNA: 3'- gaCUUcCGCUGa-CCGUGUCAgUGGuG- -5' |
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11606 | 3' | -55.2 | NC_003085.1 | + | 2075 | 0.67 | 0.625354 |
Target: 5'- -cGGAcGGUGACgcgacGaGCGCAGcCGCCGCa -3' miRNA: 3'- gaCUU-CCGCUGa----C-CGUGUCaGUGGUG- -5' |
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11606 | 3' | -55.2 | NC_003085.1 | + | 1629 | 0.68 | 0.547582 |
Target: 5'- aUGAcgcGGaCGACUGGCACA---ACCGCa -3' miRNA: 3'- gACUu--CC-GCUGACCGUGUcagUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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