Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11609 | 3' | -59.7 | NC_003085.1 | + | 48352 | 0.66 | 0.465074 |
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Target: 5'- uGGAcaucggGACGg--GCUcGCUGCCGCGGa -3' miRNA: 3'- -CCUca----CUGCagaCGA-CGGCGGCGCCg -5' |
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| 11609 | 3' | -59.7 | NC_003085.1 | + | 20033 | 0.66 | 0.474864 |
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Target: 5'- uGGAG-GACGgcaUUGCcGCCGCC-CGcGCc -3' miRNA: 3'- -CCUCaCUGCa--GACGaCGGCGGcGC-CG- -5' |
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| 11609 | 3' | -59.7 | NC_003085.1 | + | 15783 | 0.66 | 0.478809 |
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Target: 5'- gGGAGUcuGAcCGgcaugCgaauacuccuugcauUGCUgGCCGCCGUGGCc -3' miRNA: 3'- -CCUCA--CU-GCa----G---------------ACGA-CGGCGGCGCCG- -5' |
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| 11609 | 3' | -59.7 | NC_003085.1 | + | 42073 | 0.66 | 0.484756 |
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Target: 5'- cGGGGUGAagacgaugcCGaUUUGCUGCgGCUccgugucgagGUGGCg -3' miRNA: 3'- -CCUCACU---------GC-AGACGACGgCGG----------CGCCG- -5' |
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| 11609 | 3' | -59.7 | NC_003085.1 | + | 758 | 0.65 | 0.491739 |
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Target: 5'- cGGAGUu-CGUCgccGCaguugcuugcggccUGCUGCUGUGGCc -3' miRNA: 3'- -CCUCAcuGCAGa--CG--------------ACGGCGGCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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