miRNA: 3'- cuCGGUCUCAGU-------CGUGCUUGC-CGAGU- -5'
miRNA: 3'- cuCG-GUCUCaGUCGUGcUugCCGAGu -5'
miRNA: 3'- cUCGGUCucaGUCGUGCuuGCCGagu -5'
miRNA: 3'- cUCGGUCUCAG------------UCGUGCUUGCCgaGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11609 | 5' | -55.9 | NC_003085.1 | + | 10936 | 0.66 | 0.589386 |
|
Target: 5'- -uGCCGGGGUUGguguugcGCAUGAAUGcGCUCGu -3' miRNA: 3'- cuCGGUCUCAGU-------CGUGCUUGC-CGAGU- -5' |
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| 11609 | 5' | -55.9 | NC_003085.1 | + | 48285 | 0.66 | 0.601616 |
|
Target: 5'- -cGCgCAGAG-CAGCGC-GugGGCUCc -3' miRNA: 3'- cuCG-GUCUCaGUCGUGcUugCCGAGu -5' |
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| 11609 | 5' | -55.9 | NC_003085.1 | + | 36915 | 0.66 | 0.646268 |
|
Target: 5'- cGGCCAG---CGGCGCGggUGGCa-- -3' miRNA: 3'- cUCGGUCucaGUCGUGCuuGCCGagu -5' |
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| 11609 | 5' | -55.9 | NC_003085.1 | + | 18096 | 0.74 | 0.214858 |
|
Target: 5'- cGGCCGGGGUCgagcuucuugucGGCGCGGGCGGgaCGg -3' miRNA: 3'- cUCGGUCUCAG------------UCGUGCUUGCCgaGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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