Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1161 | 3' | -49.9 | NC_001132.2 | + | 76647 | 0.66 | 0.99793 |
Target: 5'- -aACGGAAC--UCCCGUaGCGUCucggGGAUa -3' miRNA: 3'- caUGCCUUGcaAGGGCA-UGUAG----UCUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 124565 | 0.66 | 0.997893 |
Target: 5'- uUACGGGAguucCGggaCCCGUGgacacguCGUCGGACg -3' miRNA: 3'- cAUGCCUU----GCaa-GGGCAU-------GUAGUCUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 150575 | 0.66 | 0.997075 |
Target: 5'- -aACGGAuGCGUcuacgaacaacUCCCGUaacgcgagaaagGCGUCGGAg -3' miRNA: 3'- caUGCCU-UGCA-----------AGGGCA------------UGUAGUCUg -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 137021 | 0.67 | 0.99526 |
Target: 5'- -gACGGAACaGUgUCCGUAC-UUGGGCg -3' miRNA: 3'- caUGCCUUG-CAaGGGCAUGuAGUCUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 9385 | 0.67 | 0.994482 |
Target: 5'- -gACGGAuccaucgagACGgcgUCCCGgACGUCAcgGACg -3' miRNA: 3'- caUGCCU---------UGCa--AGGGCaUGUAGU--CUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 72863 | 0.67 | 0.993604 |
Target: 5'- gGUugGGGACGUcCUCGUAgAcgCGGAUc -3' miRNA: 3'- -CAugCCUUGCAaGGGCAUgUa-GUCUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 22003 | 0.68 | 0.987407 |
Target: 5'- -aACGGAGCG-UCCgUGUACAugaUCAcGACg -3' miRNA: 3'- caUGCCUUGCaAGG-GCAUGU---AGU-CUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 147810 | 0.68 | 0.990284 |
Target: 5'- -gACGGAACauaCCCGUACAacauaCAGAUa -3' miRNA: 3'- caUGCCUUGcaaGGGCAUGUa----GUCUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 110750 | 0.68 | 0.991279 |
Target: 5'- aGUACGGAuaguugcgacaccauAccuaccugguuuccuCGUUCCgGUACGUCGGAg -3' miRNA: 3'- -CAUGCCU---------------U---------------GCAAGGgCAUGUAGUCUg -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 42533 | 0.69 | 0.981913 |
Target: 5'- cGUGCGG-ACGUUCgCCGUACccgAUCAa-- -3' miRNA: 3'- -CAUGCCuUGCAAG-GGCAUG---UAGUcug -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 107946 | 0.69 | 0.974789 |
Target: 5'- uGUACGGAAgcgcguuuucUGUUUCUGUACGUaccccCGGACa -3' miRNA: 3'- -CAUGCCUU----------GCAAGGGCAUGUA-----GUCUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 28596 | 0.69 | 0.974789 |
Target: 5'- -aACGGAACGUauaCUCGUACA--GGACu -3' miRNA: 3'- caUGCCUUGCAa--GGGCAUGUagUCUG- -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 92252 | 0.76 | 0.739021 |
Target: 5'- aGUACGGAuauCGUUCCCGUGgAUCGc-- -3' miRNA: 3'- -CAUGCCUu--GCAAGGGCAUgUAGUcug -5' |
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1161 | 3' | -49.9 | NC_001132.2 | + | 42575 | 0.97 | 0.065012 |
Target: 5'- uGUACGGAACGUcUCCGUACAUCAGACg -3' miRNA: 3'- -CAUGCCUUGCAaGGGCAUGUAGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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