Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11611 | 5' | -59 | NC_003085.1 | + | 22029 | 0.66 | 0.423348 |
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Target: 5'- cGUCUCuGGCcUGcGUGGCagcaGCACCa -3' miRNA: 3'- aCAGAGuCCGaACuCGCCGc---CGUGGa -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 2775 | 0.66 | 0.423348 |
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Target: 5'- cGgaagCAGGC---AGCGGCGGCGCUc -3' miRNA: 3'- aCaga-GUCCGaacUCGCCGCCGUGGa -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 28225 | 0.67 | 0.413954 |
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Target: 5'- aGUgUC-GGCcaUGGGUGGCGGCAgCg -3' miRNA: 3'- aCAgAGuCCGa-ACUCGCCGCCGUgGa -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 29414 | 0.67 | 0.386558 |
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Target: 5'- cG-CUCcaggaGGGCgcGGGCGGCGGCaauGCCg -3' miRNA: 3'- aCaGAG-----UCCGaaCUCGCCGCCG---UGGa -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 23985 | 0.67 | 0.386558 |
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Target: 5'- -uUCUCccuGGGUgaagaaGAGCGGCGGCcgACCa -3' miRNA: 3'- acAGAG---UCCGaa----CUCGCCGCCG--UGGa -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 7824 | 0.67 | 0.36897 |
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Target: 5'- ----cCAGGU--GAGCGGCGGCcaaGCCUg -3' miRNA: 3'- acagaGUCCGaaCUCGCCGCCG---UGGA- -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 33189 | 0.68 | 0.360384 |
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Target: 5'- aGUCgcgccaguagCGGGCguccugGAgGCGGCGGCGCa- -3' miRNA: 3'- aCAGa---------GUCCGaa----CU-CGCCGCCGUGga -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 32170 | 0.68 | 0.335472 |
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Target: 5'- cGUCggcCAGGCUgcgccAGUGGUGGCGCg- -3' miRNA: 3'- aCAGa--GUCCGAac---UCGCCGCCGUGga -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 27083 | 0.75 | 0.113499 |
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Target: 5'- cGUCUCGGGgUUgGGGCGGUacacguagcGGCGCCUc -3' miRNA: 3'- aCAGAGUCCgAA-CUCGCCG---------CCGUGGA- -5' |
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| 11611 | 5' | -59 | NC_003085.1 | + | 42700 | 1.07 | 0.000458 |
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Target: 5'- gUGUCUCAGGCUUGAGCGGCGGCACCUc -3' miRNA: 3'- -ACAGAGUCCGAACUCGCCGCCGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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