Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11613 | 3' | -53.6 | NC_003085.1 | + | 38067 | 0.66 | 0.820827 |
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Target: 5'- gGUgGUgCGUCG-CGGAGAcG-GCAGCg -3' miRNA: 3'- gCAgCA-GCAGCuGCUUCUcCaCGUCG- -5' |
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| 11613 | 3' | -53.6 | NC_003085.1 | + | 43431 | 0.77 | 0.240727 |
|
Target: 5'- gCGUCG-CGUCGuCGuGAGGGGUGCcGCg -3' miRNA: 3'- -GCAGCaGCAGCuGC-UUCUCCACGuCG- -5' |
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| 11613 | 3' | -53.6 | NC_003085.1 | + | 45135 | 1.13 | 0.000838 |
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Target: 5'- cCGUCGUCGUCGACGAAGAGGUGCAGCg -3' miRNA: 3'- -GCAGCAGCAGCUGCUUCUCCACGUCG- -5' |
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| 11613 | 3' | -53.6 | NC_003085.1 | + | 46884 | 0.72 | 0.48202 |
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Target: 5'- cCGUgacCG-CGUCGAUGgcGuaGGGUGCGGCa -3' miRNA: 3'- -GCA---GCaGCAGCUGCuuC--UCCACGUCG- -5' |
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| 11613 | 3' | -53.6 | NC_003085.1 | + | 47359 | 0.69 | 0.632682 |
|
Target: 5'- uGggCGcCGaUGGCGccGAGGUGCGGCg -3' miRNA: 3'- gCa-GCaGCaGCUGCuuCUCCACGUCG- -5' |
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| 11613 | 3' | -53.6 | NC_003085.1 | + | 47983 | 0.66 | 0.829975 |
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Target: 5'- gCGUCGagcaaGUCG-CGGAGGGaGgacGCGGCg -3' miRNA: 3'- -GCAGCag---CAGCuGCUUCUC-Ca--CGUCG- -5' |
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| 11613 | 3' | -53.6 | NC_003085.1 | + | 49299 | 0.67 | 0.741345 |
|
Target: 5'- gCGUCG-CGUCGGCGucccGGGAGGcgaccuccCAGCu -3' miRNA: 3'- -GCAGCaGCAGCUGC----UUCUCCac------GUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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