miRNA: 3'- cACGAGGagaAGGA-Ga--AGCUaCAGCU- -5'
miRNA: 3'- caCGAGGagaAGGAGAAGcuACAGCU- -5'
miRNA: 3'- caCGAGGaGAAGGAGAAGCuaCaGCu -5'
miRNA: 3'- caCGa----GGAGAAGGAgaAGCUaCAGCU- -5'
miRNA: 3'- -CACGAGGAGAAGGAGAAGCUACAGCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11613 | 5' | -51.7 | NC_003085.1 | + | 47444 | 0.66 | 0.873153 |
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Target: 5'- cUGCUCCaugUCCUgCgcgUCGAaGUCGAa -3' miRNA: 3'- cACGAGGagaAGGA-Ga--AGCUaCAGCU- -5' |
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| 11613 | 5' | -51.7 | NC_003085.1 | + | 16292 | 0.66 | 0.873153 |
|
Target: 5'- -cGCUCC---UCCUCUUC--UGUCGGg -3' miRNA: 3'- caCGAGGagaAGGAGAAGcuACAGCU- -5' |
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| 11613 | 5' | -51.7 | NC_003085.1 | + | 35524 | 0.66 | 0.856394 |
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Target: 5'- -cGCUCCgCUUCUUCUUCGgcG-CGc -3' miRNA: 3'- caCGAGGaGAAGGAGAAGCuaCaGCu -5' |
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| 11613 | 5' | -51.7 | NC_003085.1 | + | 45431 | 0.69 | 0.715647 |
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Target: 5'- -aGCgacagCCUCUUCCU--UCGA-GUCGAa -3' miRNA: 3'- caCGa----GGAGAAGGAgaAGCUaCAGCU- -5' |
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| 11613 | 5' | -51.7 | NC_003085.1 | + | 45172 | 1.1 | 0.001707 |
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Target: 5'- cGUGCUCCUCUUCCUCUUCGAUGUCGAa -3' miRNA: 3'- -CACGAGGAGAAGGAGAAGCUACAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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