Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11615 | 3' | -57.2 | NC_003085.1 | + | 23791 | 0.66 | 0.614244 |
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Target: 5'- gCGAAuucGUCCg-GCCGGuagucgcAGUCCGCagCACCg -3' miRNA: 3'- -GCUU---CAGGagCGGCC-------UCAGGUG--GUGG- -5' |
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| 11615 | 3' | -57.2 | NC_003085.1 | + | 5701 | 0.66 | 0.615323 |
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Target: 5'- -uGAGUCCaaGCCcGcGcCCACCACCg -3' miRNA: 3'- gcUUCAGGagCGGcCuCaGGUGGUGG- -5' |
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| 11615 | 3' | -57.2 | NC_003085.1 | + | 45059 | 0.66 | 0.615323 |
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Target: 5'- ----cUCCUcCGCCGGcuGUCCGguauCCGCCg -3' miRNA: 3'- gcuucAGGA-GCGGCCu-CAGGU----GGUGG- -5' |
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| 11615 | 3' | -57.2 | NC_003085.1 | + | 29666 | 0.66 | 0.615323 |
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Target: 5'- aGAAGUCa-UGUCGGGcGUCCACC-UCg -3' miRNA: 3'- gCUUCAGgaGCGGCCU-CAGGUGGuGG- -5' |
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| 11615 | 3' | -57.2 | NC_003085.1 | + | 39308 | 0.66 | 0.626126 |
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Target: 5'- --cGGUCCUCG-CGGuacUCC-CCGCCg -3' miRNA: 3'- gcuUCAGGAGCgGCCuc-AGGuGGUGG- -5' |
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| 11615 | 3' | -57.2 | NC_003085.1 | + | 24758 | 0.66 | 0.626126 |
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Target: 5'- gCGAAG-CCgccgaUGCCGccGUCCAgCACCa -3' miRNA: 3'- -GCUUCaGGa----GCGGCcuCAGGUgGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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