Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11616 | 3' | -53.6 | NC_003085.1 | + | 30632 | 0.66 | 0.802411 |
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Target: 5'- aGUCGag-CcCACUCAacuaccGUGGCGAGUCg -3' miRNA: 3'- gCAGCagaGcGUGAGU------CACUGCUCAG- -5' |
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| 11616 | 3' | -53.6 | NC_003085.1 | + | 32701 | 0.66 | 0.792618 |
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Target: 5'- gCGUCGUCUgguaGCGCUCGGguaGCGAcaacaaccGUCc -3' miRNA: 3'- -GCAGCAGAg---CGUGAGUCac-UGCU--------CAG- -5' |
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| 11616 | 3' | -53.6 | NC_003085.1 | + | 12408 | 0.66 | 0.791629 |
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Target: 5'- aCGUCGUCggCGCcagcgcgguacagGC-CGGUGucCGGGUCg -3' miRNA: 3'- -GCAGCAGa-GCG-------------UGaGUCACu-GCUCAG- -5' |
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| 11616 | 3' | -53.6 | NC_003085.1 | + | 42777 | 0.69 | 0.587252 |
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Target: 5'- gCGUCGgCUUGagcCUCGGUGACGGGa- -3' miRNA: 3'- -GCAGCaGAGCgu-GAGUCACUGCUCag -5' |
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| 11616 | 3' | -53.6 | NC_003085.1 | + | 47385 | 0.7 | 0.547749 |
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Target: 5'- gCGUCGUagccUUCGCGCUCGGccuccuggaugacccUGAUGaAGUCg -3' miRNA: 3'- -GCAGCA----GAGCGUGAGUC---------------ACUGC-UCAG- -5' |
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| 11616 | 3' | -53.6 | NC_003085.1 | + | 47915 | 1.11 | 0.001097 |
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Target: 5'- gCGUCGUCUCGCACUCAGUGACGAGUCg -3' miRNA: 3'- -GCAGCAGAGCGUGAGUCACUGCUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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