Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1163 | 5' | -53.3 | NC_001132.2 | + | 2150 | 0.66 | 0.981221 |
Target: 5'- gGAACGAG-CggcaGCGAGgGCGCg- -3' miRNA: 3'- gUUUGCUCaGauugCGCUCgCGCGgg -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 10137 | 0.66 | 0.97904 |
Target: 5'- ---cCGAGUUgaAACGCaGGGCGCgauggcuuauGCCCa -3' miRNA: 3'- guuuGCUCAGa-UUGCG-CUCGCG----------CGGG- -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 52396 | 0.66 | 0.971372 |
Target: 5'- aCAAAgGGGUUguuUAACGCGAGCaaCGUCg -3' miRNA: 3'- -GUUUgCUCAG---AUUGCGCUCGc-GCGGg -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 144350 | 0.67 | 0.965252 |
Target: 5'- aGAAgGAuUCUAGCGCGGauuCGUGUCCg -3' miRNA: 3'- gUUUgCUcAGAUUGCGCUc--GCGCGGG- -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 160768 | 0.67 | 0.961871 |
Target: 5'- ---uUGAGUUauACGCGGGacaaGCGCCg -3' miRNA: 3'- guuuGCUCAGauUGCGCUCg---CGCGGg -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 127312 | 0.67 | 0.954437 |
Target: 5'- aCGGugGcAGUCUcGACGCGguaAGUcCGCCCu -3' miRNA: 3'- -GUUugC-UCAGA-UUGCGC---UCGcGCGGG- -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 157633 | 0.68 | 0.94608 |
Target: 5'- -cGACGuGUCgauuaacGugGCGAucGCGaCGCCCg -3' miRNA: 3'- guUUGCuCAGa------UugCGCU--CGC-GCGGG- -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 37913 | 0.68 | 0.936774 |
Target: 5'- aCGAACGGGccUCUGACGaCGAccugGCG-GCCUg -3' miRNA: 3'- -GUUUGCUC--AGAUUGC-GCU----CGCgCGGG- -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 44826 | 0.7 | 0.85307 |
Target: 5'- -cGACGGG-C--ACGCGA-CGCGCCCa -3' miRNA: 3'- guUUGCUCaGauUGCGCUcGCGCGGG- -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 92292 | 0.73 | 0.730147 |
Target: 5'- --cACGAGUUgcguccguaacgucGACGCGAGCaGCGCCa -3' miRNA: 3'- guuUGCUCAGa-------------UUGCGCUCG-CGCGGg -5' |
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1163 | 5' | -53.3 | NC_001132.2 | + | 46085 | 1.12 | 0.003589 |
Target: 5'- uCAAACGAGUCUAACGCGAGCGCGCCCg -3' miRNA: 3'- -GUUUGCUCAGAUUGCGCUCGCGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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