Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11641 | 5' | -52 | NC_003094.2 | + | 84300 | 0.7 | 0.883785 |
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Target: 5'- -aUCAACgaACUGAGCGCAuuauguucuuCGugCGGCg -3' miRNA: 3'- caAGUUGg-UGGCUUGCGU----------GUugGCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 102894 | 0.7 | 0.876473 |
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Target: 5'- --gCAGCCgcgACCGAACGUuauaaAGCUGGCu -3' miRNA: 3'- caaGUUGG---UGGCUUGCGug---UUGGCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 111936 | 0.73 | 0.752946 |
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Target: 5'- --cUAACCACacauaCGAAgGUACAACUGGCa -3' miRNA: 3'- caaGUUGGUG-----GCUUgCGUGUUGGCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 39318 | 0.73 | 0.752946 |
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Target: 5'- -aUCuuCCGCCGAAgGCAauuuAUCGGCg -3' miRNA: 3'- caAGuuGGUGGCUUgCGUgu--UGGCCG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 81701 | 0.74 | 0.691661 |
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Target: 5'- -gUCGACCGcCCGAACGUcuACGGCCGccGCc -3' miRNA: 3'- caAGUUGGU-GGCUUGCG--UGUUGGC--CG- -5' |
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| 11641 | 5' | -52 | NC_003094.2 | + | 58581 | 1.13 | 0.003326 |
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Target: 5'- aGUUCAACCACCGAACGCACAACCGGCa -3' miRNA: 3'- -CAAGUUGGUGGCUUGCGUGUUGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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