miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11642 5' -45.4 NC_003094.2 + 72974 0.66 0.999912
Target:  5'- aAGCAUGCcGUggcgCAUGAgCACGUUu- -3'
miRNA:   3'- -UCGUACGaCAaa--GUAUUaGUGCAGuu -5'
11642 5' -45.4 NC_003094.2 + 32039 0.68 0.999457
Target:  5'- aGGCAUGg-GUUUCAUAuaCAUGUCGu -3'
miRNA:   3'- -UCGUACgaCAAAGUAUuaGUGCAGUu -5'
11642 5' -45.4 NC_003094.2 + 44358 0.68 0.999146
Target:  5'- ----aGUUGUUUCAuugUAAUCAUGUCGAa -3'
miRNA:   3'- ucguaCGACAAAGU---AUUAGUGCAGUU- -5'
11642 5' -45.4 NC_003094.2 + 32447 0.69 0.998368
Target:  5'- aAGUAUGCaGUaUCGUugaaacacgucagGGUCGCGUCAAu -3'
miRNA:   3'- -UCGUACGaCAaAGUA-------------UUAGUGCAGUU- -5'
11642 5' -45.4 NC_003094.2 + 25121 0.71 0.988174
Target:  5'- uGUAUGCUGUaaucgUUCAUAGUCAUGg--- -3'
miRNA:   3'- uCGUACGACA-----AAGUAUUAGUGCaguu -5'
11642 5' -45.4 NC_003094.2 + 73098 1.07 0.029671
Target:  5'- uAGCAUGCUGUUUCAUAAUCACGUCAAc -3'
miRNA:   3'- -UCGUACGACAAAGUAUUAGUGCAGUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.