Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11642 | 5' | -45.4 | NC_003094.2 | + | 72974 | 0.66 | 0.999912 |
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Target: 5'- aAGCAUGCcGUggcgCAUGAgCACGUUu- -3' miRNA: 3'- -UCGUACGaCAaa--GUAUUaGUGCAGuu -5' |
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| 11642 | 5' | -45.4 | NC_003094.2 | + | 32039 | 0.68 | 0.999457 |
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Target: 5'- aGGCAUGg-GUUUCAUAuaCAUGUCGu -3' miRNA: 3'- -UCGUACgaCAAAGUAUuaGUGCAGUu -5' |
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| 11642 | 5' | -45.4 | NC_003094.2 | + | 44358 | 0.68 | 0.999146 |
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Target: 5'- ----aGUUGUUUCAuugUAAUCAUGUCGAa -3' miRNA: 3'- ucguaCGACAAAGU---AUUAGUGCAGUU- -5' |
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| 11642 | 5' | -45.4 | NC_003094.2 | + | 32447 | 0.69 | 0.998368 |
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Target: 5'- aAGUAUGCaGUaUCGUugaaacacgucagGGUCGCGUCAAu -3' miRNA: 3'- -UCGUACGaCAaAGUA-------------UUAGUGCAGUU- -5' |
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| 11642 | 5' | -45.4 | NC_003094.2 | + | 25121 | 0.71 | 0.988174 |
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Target: 5'- uGUAUGCUGUaaucgUUCAUAGUCAUGg--- -3' miRNA: 3'- uCGUACGACA-----AAGUAUUAGUGCaguu -5' |
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| 11642 | 5' | -45.4 | NC_003094.2 | + | 73098 | 1.07 | 0.029671 |
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Target: 5'- uAGCAUGCUGUUUCAUAAUCACGUCAAc -3' miRNA: 3'- -UCGUACGACAAAGUAUUAGUGCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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