Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11645 | 3' | -54.9 | NC_003094.2 | + | 7746 | 0.66 | 0.930685 |
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Target: 5'- cGUCGUCUGUG-UCAUCGUUCaAACa -3' miRNA: 3'- cUAGCAGACGCuGGUGGCGAGcUUGc -5' |
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| 11645 | 3' | -54.9 | NC_003094.2 | + | 113728 | 0.66 | 0.914012 |
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Target: 5'- cGUCGUCUGUGACaagUACgGCUgCGGcuACGg -3' miRNA: 3'- cUAGCAGACGCUG---GUGgCGA-GCU--UGC- -5' |
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| 11645 | 3' | -54.9 | NC_003094.2 | + | 81714 | 0.68 | 0.8668 |
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Target: 5'- --aCGUCUaCGGCCGCCGCcgccgccUGAGCGu -3' miRNA: 3'- cuaGCAGAcGCUGGUGGCGa------GCUUGC- -5' |
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| 11645 | 3' | -54.9 | NC_003094.2 | + | 81675 | 0.68 | 0.835002 |
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Target: 5'- --gCGUC-GaCGACCGCCGCUaGAACu -3' miRNA: 3'- cuaGCAGaC-GCUGGUGGCGAgCUUGc -5' |
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| 11645 | 3' | -54.9 | NC_003094.2 | + | 81650 | 0.71 | 0.693547 |
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Target: 5'- -cUCGUCUaCcGCCACCGCUgGAACu -3' miRNA: 3'- cuAGCAGAcGcUGGUGGCGAgCUUGc -5' |
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| 11645 | 3' | -54.9 | NC_003094.2 | + | 43659 | 0.74 | 0.519978 |
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Target: 5'- -uUCGUC-GCGGCCACUGCU-GAACa -3' miRNA: 3'- cuAGCAGaCGCUGGUGGCGAgCUUGc -5' |
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| 11645 | 3' | -54.9 | NC_003094.2 | + | 127110 | 0.78 | 0.349867 |
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Target: 5'- cGAUCG-UUGCGcACCACCGUUCGuGCGg -3' miRNA: 3'- -CUAGCaGACGC-UGGUGGCGAGCuUGC- -5' |
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| 11645 | 3' | -54.9 | NC_003094.2 | + | 81535 | 0.87 | 0.100417 |
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Target: 5'- ---nGUCUGCGACCACCGCcCGAACGc -3' miRNA: 3'- cuagCAGACGCUGGUGGCGaGCUUGC- -5' |
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| 11645 | 3' | -54.9 | NC_003094.2 | + | 81510 | 1.08 | 0.003743 |
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Target: 5'- aGAUCGUCUGCGACCACCGCUCGAACGu -3' miRNA: 3'- -CUAGCAGACGCUGGUGGCGAGCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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