Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11652 | 3' | -55.1 | NC_003102.1 | + | 93496 | 0.66 | 0.949138 |
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Target: 5'- cUCaCGuacUGCAcGUCgGCGUCGGUGGGa -3' miRNA: 3'- -AG-GCuacAUGUcUAG-CGCAGCCGCCC- -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 37122 | 0.66 | 0.949138 |
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Target: 5'- gUUCGGuaUGUACGGAUCu--UCGGCGGu -3' miRNA: 3'- -AGGCU--ACAUGUCUAGcgcAGCCGCCc -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 71916 | 0.66 | 0.944833 |
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Target: 5'- gCCGAgcaGCGuGGUCGcCGUCGGCGa- -3' miRNA: 3'- aGGCUacaUGU-CUAGC-GCAGCCGCcc -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 109788 | 0.66 | 0.930519 |
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Target: 5'- -gCGAcGUACAGAgacgaUGUGUCGGCGa- -3' miRNA: 3'- agGCUaCAUGUCUa----GCGCAGCCGCcc -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 875 | 0.66 | 0.930519 |
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Target: 5'- cCCG-UGUAUAGAggCGCGUCGGg--- -3' miRNA: 3'- aGGCuACAUGUCUa-GCGCAGCCgccc -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 107479 | 0.67 | 0.914085 |
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Target: 5'- gCUGAgcaaGUACGGAUUGuCGUCGGCc-- -3' miRNA: 3'- aGGCUa---CAUGUCUAGC-GCAGCCGccc -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 84790 | 0.68 | 0.860159 |
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Target: 5'- -aCGA---ACAGGUCGuCGugaUCGGCGGGa -3' miRNA: 3'- agGCUacaUGUCUAGC-GC---AGCCGCCC- -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 103079 | 0.68 | 0.852451 |
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Target: 5'- aUUCGAcGUuuGGGUCGCGUCcGCGGu -3' miRNA: 3'- -AGGCUaCAugUCUAGCGCAGcCGCCc -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 63474 | 0.73 | 0.616528 |
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Target: 5'- gCCGuUGUuCGGAUCGCGUCGucuGCGGu -3' miRNA: 3'- aGGCuACAuGUCUAGCGCAGC---CGCCc -5' |
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| 11652 | 3' | -55.1 | NC_003102.1 | + | 17495 | 1.11 | 0.003039 |
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Target: 5'- aUCCGAUGUACAGAUCGCGUCGGCGGGu -3' miRNA: 3'- -AGGCUACAUGUCUAGCGCAGCCGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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