Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11653 | 3' | -55.8 | NC_003102.1 | + | 133740 | 0.69 | 0.753905 |
Target: 5'- cCGCGUCGcAGcCGCCguaGUGUCGcCGCa- -3' miRNA: 3'- aGCGCAGC-UC-GCGG---UACAGUuGCGcg -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 93548 | 0.7 | 0.72371 |
Target: 5'- uUCGUGUcuguacaCGAGCaCCGUGUCgAAUGUGCc -3' miRNA: 3'- -AGCGCA-------GCUCGcGGUACAG-UUGCGCG- -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 17482 | 0.7 | 0.704812 |
Target: 5'- aUCGCGUCG-GCgGgUAUGUCGaugGCGgGCa -3' miRNA: 3'- -AGCGCAGCuCG-CgGUACAGU---UGCgCG- -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 1274 | 0.71 | 0.674548 |
Target: 5'- aUCGCGUCGAGCGUgauagccgaaagCAgcgagGUCAuacgaaacaACGCGg -3' miRNA: 3'- -AGCGCAGCUCGCG------------GUa----CAGU---------UGCGCg -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 127179 | 0.71 | 0.664379 |
Target: 5'- uUCGCGgccacguccauUCGaAGCGCCA-GUCuGCGCGg -3' miRNA: 3'- -AGCGC-----------AGC-UCGCGGUaCAGuUGCGCg -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 128583 | 0.71 | 0.643972 |
Target: 5'- gCGCGUUGAGCGCgCuagacaGACGCGUc -3' miRNA: 3'- aGCGCAGCUCGCG-Guacag-UUGCGCG- -5' |
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11653 | 3' | -55.8 | NC_003102.1 | + | 20124 | 0.99 | 0.013433 |
Target: 5'- cUCGCGUCGAGCGCCAUGUacACGCGCg -3' miRNA: 3'- -AGCGCAGCUCGCGGUACAguUGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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