Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11659 | 3' | -56 | NC_003102.1 | + | 100147 | 0.66 | 0.914085 |
Target: 5'- -uUGGUCGC-AUCGUGGUcggcGACGGUCGc -3' miRNA: 3'- auGCCAGCGcUGGUGCCA----UUGCCAGC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 113006 | 0.66 | 0.895543 |
Target: 5'- gGCGGUgGUGGuguCgGCGGUGGCGG-Ca -3' miRNA: 3'- aUGCCAgCGCU---GgUGCCAUUGCCaGc -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 102035 | 0.66 | 0.895543 |
Target: 5'- aGCGG-CgGCGGugGCGGUGGCGG-CGg -3' miRNA: 3'- aUGCCaG-CGCUggUGCCAUUGCCaGC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 17744 | 0.67 | 0.882042 |
Target: 5'- gGCGG-CgGCGGCgGCGGUGcCGGUg- -3' miRNA: 3'- aUGCCaG-CGCUGgUGCCAUuGCCAgc -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 1660 | 0.67 | 0.874961 |
Target: 5'- -gUGGUgGCGGCgGCGGUAAaauuUGuGUCGg -3' miRNA: 3'- auGCCAgCGCUGgUGCCAUU----GC-CAGC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 119060 | 0.68 | 0.811087 |
Target: 5'- aGCGauagucGUCGUGAUCGCGGUGGCGugcuGUUGg -3' miRNA: 3'- aUGC------CAGCGCUGGUGCCAUUGC----CAGC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 72718 | 0.69 | 0.775067 |
Target: 5'- aACGGUgGUGGaaAUGGUAGCGG-CGg -3' miRNA: 3'- aUGCCAgCGCUggUGCCAUUGCCaGC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 114985 | 0.71 | 0.657199 |
Target: 5'- aACGG-CgGCGACaGCGGUGGCGGUgGc -3' miRNA: 3'- aUGCCaG-CGCUGgUGCCAUUGCCAgC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 32404 | 0.72 | 0.616528 |
Target: 5'- gACGGcUGCGGCUGCGGcggcAACGGUUGa -3' miRNA: 3'- aUGCCaGCGCUGGUGCCa---UUGCCAGC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 103087 | 0.72 | 0.616528 |
Target: 5'- uUugGGUCGCGuCCGCGGUcauguccacGAUGGgacCGa -3' miRNA: 3'- -AugCCAGCGCuGGUGCCA---------UUGCCa--GC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 59511 | 0.72 | 0.616528 |
Target: 5'- aGCGGcgaCGCGACgGCGGcAACGGUUc -3' miRNA: 3'- aUGCCa--GCGCUGgUGCCaUUGCCAGc -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 103242 | 0.72 | 0.602313 |
Target: 5'- cGCGGaCGCGACCcaaacgucgaaucUGGUGGCGGUCc -3' miRNA: 3'- aUGCCaGCGCUGGu------------GCCAUUGCCAGc -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 16452 | 0.73 | 0.57604 |
Target: 5'- cGCaGUCGCGGCgGCGGUccAACaGGUCa -3' miRNA: 3'- aUGcCAGCGCUGgUGCCA--UUG-CCAGc -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 134373 | 0.73 | 0.526355 |
Target: 5'- aGCGG-CgGCGGCgGCGGcGGCGGUCGc -3' miRNA: 3'- aUGCCaG-CGCUGgUGCCaUUGCCAGC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 15089 | 0.81 | 0.198545 |
Target: 5'- -uCGGUCGCGaACCgGCGGUAaaACGGUCGa -3' miRNA: 3'- auGCCAGCGC-UGG-UGCCAU--UGCCAGC- -5' |
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11659 | 3' | -56 | NC_003102.1 | + | 32608 | 1.06 | 0.004727 |
Target: 5'- cUACGGUCGCGACCACGGUAACGGUCGc -3' miRNA: 3'- -AUGCCAGCGCUGGUGCCAUUGCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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