Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1166 | 5' | -45.6 | NC_001132.2 | + | 16377 | 0.66 | 0.999997 |
Target: 5'- -aUAAACCACauGACcGACGACGUGUg -3' miRNA: 3'- ccAUUUGGUGccUUGcUUGUUGCAUA- -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 98064 | 0.66 | 0.999992 |
Target: 5'- uGGUAacGACCGcCGGAGuuccauGCAGCGUGUc -3' miRNA: 3'- -CCAU--UUGGU-GCCUUgcu---UGUUGCAUA- -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 143106 | 0.67 | 0.999983 |
Target: 5'- uGGUuuguuGCCACGGcuAACGuGACAcACGUGUc -3' miRNA: 3'- -CCAuu---UGGUGCC--UUGC-UUGU-UGCAUA- -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 89037 | 0.67 | 0.999943 |
Target: 5'- --cAAACCcacGCGGGACGAGucguUGACGUGUa -3' miRNA: 3'- ccaUUUGG---UGCCUUGCUU----GUUGCAUA- -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 12686 | 0.67 | 0.999943 |
Target: 5'- cGUAGACgACGuagacGACGAugACAACGUAUa -3' miRNA: 3'- cCAUUUGgUGCc----UUGCU--UGUUGCAUA- -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 147386 | 0.67 | 0.999938 |
Target: 5'- -uUGAACUugGGAAaCGAgagauguacguaucGCAACGUGg -3' miRNA: 3'- ccAUUUGGugCCUU-GCU--------------UGUUGCAUa -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 93888 | 0.68 | 0.9999 |
Target: 5'- --cAAACUagaaACGGAagAUGAACAACGUAc -3' miRNA: 3'- ccaUUUGG----UGCCU--UGCUUGUUGCAUa -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 141240 | 0.69 | 0.999781 |
Target: 5'- -uUAAACgGUGGAACGGAUAACGUu- -3' miRNA: 3'- ccAUUUGgUGCCUUGCUUGUUGCAua -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 35869 | 0.69 | 0.999552 |
Target: 5'- ----cACCACGGAcgcgaaggccACGGugAACGUAUu -3' miRNA: 3'- ccauuUGGUGCCU----------UGCUugUUGCAUA- -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 2161 | 0.75 | 0.965319 |
Target: 5'- cGGaauACUACGGAACGAGCGGCa--- -3' miRNA: 3'- -CCauuUGGUGCCUUGCUUGUUGcaua -5' |
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1166 | 5' | -45.6 | NC_001132.2 | + | 134694 | 1.09 | 0.036415 |
Target: 5'- uGGUAAACCACGGAACGAACAACGUAUc -3' miRNA: 3'- -CCAUUUGGUGCCUUGCUUGUUGCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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