Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11660 | 3' | -47.5 | NC_003102.1 | + | 39182 | 0.66 | 0.999511 |
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Target: 5'- gAGGCGUUUgUGUUuuuGAGACAGGCGu- -3' miRNA: 3'- -UUUGCAAGgGCAGu--CUCUGUUUGUug -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 37755 | 0.67 | 0.998586 |
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Target: 5'- cAAACGguuUCCCGUC-GAGAuCAAACu-- -3' miRNA: 3'- -UUUGCa--AGGGCAGuCUCU-GUUUGuug -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 22477 | 0.67 | 0.998283 |
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Target: 5'- cGAAuCGauaCCGUUAGAGAuCAGGCAACu -3' miRNA: 3'- -UUU-GCaagGGCAGUCUCU-GUUUGUUG- -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 65335 | 0.67 | 0.998283 |
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Target: 5'- uGACGU--UCGUUAGAGACuuuugAAACAGCa -3' miRNA: 3'- uUUGCAagGGCAGUCUCUG-----UUUGUUG- -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 134910 | 0.67 | 0.998283 |
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Target: 5'- cGACGgucgcaUCCuCGUCgagcAGAGACGAACAcaGCa -3' miRNA: 3'- uUUGCa-----AGG-GCAG----UCUCUGUUUGU--UG- -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 92100 | 0.68 | 0.997031 |
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Target: 5'- cGGCGgcgCCggCGUCGGAGGCGAACu-- -3' miRNA: 3'- uUUGCaa-GG--GCAGUCUCUGUUUGuug -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 96972 | 0.71 | 0.975556 |
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Target: 5'- cGGCGUUCCaCGcguacaUCGGAGACAGuugcuCGACg -3' miRNA: 3'- uUUGCAAGG-GC------AGUCUCUGUUu----GUUG- -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 87267 | 0.72 | 0.966361 |
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Target: 5'- -cACGUucggcUCCCGUCAGAGccggaauauaaGCGuACAACg -3' miRNA: 3'- uuUGCA-----AGGGCAGUCUC-----------UGUuUGUUG- -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 71910 | 0.73 | 0.936059 |
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Target: 5'- cAGCGUggUCgCCGUCGGcGACAagGACAGCg -3' miRNA: 3'- uUUGCA--AG-GGCAGUCuCUGU--UUGUUG- -5' |
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| 11660 | 3' | -47.5 | NC_003102.1 | + | 33290 | 1.09 | 0.015237 |
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Target: 5'- gAAACGUUCCCGUCAGAGACAAACAACg -3' miRNA: 3'- -UUUGCAAGGGCAGUCUCUGUUUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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