Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11662 | 5' | -46.9 | NC_003102.1 | + | 11304 | 0.66 | 0.999685 |
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Target: 5'- -gUGGAuugGCGAcGAACAuguuCGUUgCGUAUa -3' miRNA: 3'- aaACCU---UGCU-CUUGUu---GCAAgGCAUG- -5' |
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| 11662 | 5' | -46.9 | NC_003102.1 | + | 63571 | 0.66 | 0.9996 |
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Target: 5'- --aGGAaucgacGCGGGAGCGGCuccUCCGUAa -3' miRNA: 3'- aaaCCU------UGCUCUUGUUGca-AGGCAUg -5' |
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| 11662 | 5' | -46.9 | NC_003102.1 | + | 83005 | 0.67 | 0.999495 |
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Target: 5'- -gUGGAcACGA--GCGACGUgucgUCGUACg -3' miRNA: 3'- aaACCU-UGCUcuUGUUGCAa---GGCAUG- -5' |
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| 11662 | 5' | -46.9 | NC_003102.1 | + | 97432 | 0.67 | 0.999368 |
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Target: 5'- cUUGGucaacauGCGGGGACAuuuGCGUUgcaCGUACu -3' miRNA: 3'- aAACCu------UGCUCUUGU---UGCAAg--GCAUG- -5' |
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| 11662 | 5' | -46.9 | NC_003102.1 | + | 107307 | 0.67 | 0.99903 |
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Target: 5'- ---cGGGCGAGGcCGACGacaaUCCGUACu -3' miRNA: 3'- aaacCUUGCUCUuGUUGCa---AGGCAUG- -5' |
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| 11662 | 5' | -46.9 | NC_003102.1 | + | 66140 | 0.68 | 0.998809 |
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Target: 5'- -gUGGAcaaguugcugAUGGGAcACAGCGUUUCGUGu -3' miRNA: 3'- aaACCU----------UGCUCU-UGUUGCAAGGCAUg -5' |
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| 11662 | 5' | -46.9 | NC_003102.1 | + | 100009 | 0.68 | 0.998237 |
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Target: 5'- aUUUGcGAACGAGAuCAAUGcaaCGUGCg -3' miRNA: 3'- -AAAC-CUUGCUCUuGUUGCaagGCAUG- -5' |
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| 11662 | 5' | -46.9 | NC_003102.1 | + | 77129 | 0.7 | 0.992092 |
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Target: 5'- --aGGGACGAGAACGACGag-UGUAa -3' miRNA: 3'- aaaCCUUGCUCUUGUUGCaagGCAUg -5' |
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| 11662 | 5' | -46.9 | NC_003102.1 | + | 42129 | 1.09 | 0.018602 |
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Target: 5'- gUUUGGAACGAGAACAACGUUCCGUACg -3' miRNA: 3'- -AAACCUUGCUCUUGUUGCAAGGCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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