Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11664 | 3' | -51.7 | NC_003102.1 | + | 6598 | 0.66 | 0.983174 |
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Target: 5'- cCGAugguGACGUUGGCgcugGCGCUGcCGAGAc -3' miRNA: 3'- -GUUuu--UUGCGGUCGa---CGUGGC-GCUCU- -5' |
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| 11664 | 3' | -51.7 | NC_003102.1 | + | 29145 | 0.66 | 0.978806 |
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Target: 5'- --uAAGACGCgAGCgcGCGCCGCa--- -3' miRNA: 3'- guuUUUUGCGgUCGa-CGUGGCGcucu -5' |
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| 11664 | 3' | -51.7 | NC_003102.1 | + | 530 | 0.67 | 0.960716 |
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Target: 5'- aGAAGGGCGgCGGUUGUcCCGUGAuGAa -3' miRNA: 3'- gUUUUUUGCgGUCGACGuGGCGCU-CU- -5' |
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| 11664 | 3' | -51.7 | NC_003102.1 | + | 127605 | 0.67 | 0.956896 |
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Target: 5'- -cGAAAGCGCUuaAGUcugUGUACaCGCGGGAc -3' miRNA: 3'- guUUUUUGCGG--UCG---ACGUG-GCGCUCU- -5' |
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| 11664 | 3' | -51.7 | NC_003102.1 | + | 43348 | 0.68 | 0.943947 |
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Target: 5'- --uGGAACGCCGGCaUGUucagAUUGUGAGAg -3' miRNA: 3'- guuUUUUGCGGUCG-ACG----UGGCGCUCU- -5' |
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| 11664 | 3' | -51.7 | NC_003102.1 | + | 35551 | 0.71 | 0.853063 |
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Target: 5'- -------aGCCAaGuCUGCGCUGCGAGAc -3' miRNA: 3'- guuuuuugCGGU-C-GACGUGGCGCUCU- -5' |
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| 11664 | 3' | -51.7 | NC_003102.1 | + | 129127 | 0.74 | 0.710549 |
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Target: 5'- -cAGAAGCGgCAGCUGC-CgGCGGGGu -3' miRNA: 3'- guUUUUUGCgGUCGACGuGgCGCUCU- -5' |
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| 11664 | 3' | -51.7 | NC_003102.1 | + | 70733 | 0.74 | 0.689545 |
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Target: 5'- aCGAAGcGCGCCAGCUG-ACCGuCGAcGAc -3' miRNA: 3'- -GUUUUuUGCGGUCGACgUGGC-GCU-CU- -5' |
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| 11664 | 3' | -51.7 | NC_003102.1 | + | 46238 | 1.1 | 0.005221 |
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Target: 5'- aCAAAAAACGCCAGCUGCACCGCGAGAa -3' miRNA: 3'- -GUUUUUUGCGGUCGACGUGGCGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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