Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11664 | 5' | -55.8 | NC_003102.1 | + | 134953 | 0.66 | 0.912752 |
Target: 5'- uGCgaaCCGUCGccgcucaacacGCCGACGCCGg-- -3' miRNA: 3'- -CGa--GGCAGCcauu-------CGGCUGCGGCaaa -5' |
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11664 | 5' | -55.8 | NC_003102.1 | + | 107303 | 0.66 | 0.897783 |
Target: 5'- ---gCGUCGGgcgAGGCCGACGacaauCCGUa- -3' miRNA: 3'- cgagGCAGCCa--UUCGGCUGC-----GGCAaa -5' |
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11664 | 5' | -55.8 | NC_003102.1 | + | 39248 | 0.66 | 0.884281 |
Target: 5'- aGUcgCCGUCGGUuuGAGaCCGAgGaCCGUg- -3' miRNA: 3'- -CGa-GGCAGCCA--UUC-GGCUgC-GGCAaa -5' |
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11664 | 5' | -55.8 | NC_003102.1 | + | 22696 | 0.69 | 0.757716 |
Target: 5'- cGCUaCUGUUGGc--GCCGAUGCCGa-- -3' miRNA: 3'- -CGA-GGCAGCCauuCGGCUGCGGCaaa -5' |
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11664 | 5' | -55.8 | NC_003102.1 | + | 32709 | 0.69 | 0.738291 |
Target: 5'- aUUCUGUugCGGUucGCCGuCGCCGUUc -3' miRNA: 3'- cGAGGCA--GCCAuuCGGCuGCGGCAAa -5' |
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11664 | 5' | -55.8 | NC_003102.1 | + | 63668 | 0.72 | 0.606193 |
Target: 5'- aGCUCCGgaaCGGaUAcGUCGACGCCGc-- -3' miRNA: 3'- -CGAGGCa--GCC-AUuCGGCUGCGGCaaa -5' |
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11664 | 5' | -55.8 | NC_003102.1 | + | 79769 | 0.72 | 0.59594 |
Target: 5'- -aUCCGUCuuUGAGCaCGACGCCGUc- -3' miRNA: 3'- cgAGGCAGccAUUCG-GCUGCGGCAaa -5' |
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11664 | 5' | -55.8 | NC_003102.1 | + | 129014 | 0.77 | 0.331478 |
Target: 5'- uGCUCCGUCGGc--GCCGGcCGCCGc-- -3' miRNA: 3'- -CGAGGCAGCCauuCGGCU-GCGGCaaa -5' |
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11664 | 5' | -55.8 | NC_003102.1 | + | 46204 | 1.08 | 0.003462 |
Target: 5'- gGCUCCGUCGGUAAGCCGACGCCGUUUa -3' miRNA: 3'- -CGAGGCAGCCAUUCGGCUGCGGCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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