miRNA display CGI


Results 1 - 20 of 21 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11666 5' -49.3 NC_003102.1 + 66745 0.66 0.997796
Target:  5'- -cGAGACCGACGAUgacgaCUgcgGCgGCGAc- -3'
miRNA:   3'- uaCUUUGGCUGCUAg----GAa--UG-CGCUag -5'
11666 5' -49.3 NC_003102.1 + 131434 0.66 0.997632
Target:  5'- uUGAGACCGugcACGAUCaccgaagucuCGCGGUUg -3'
miRNA:   3'- uACUUUGGC---UGCUAGgaau------GCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 35578 0.66 0.997368
Target:  5'- -cGAAGCCGACGAgauaUCgc-CGCGAg- -3'
miRNA:   3'- uaCUUUGGCUGCUa---GGaauGCGCUag -5'
11666 5' -49.3 NC_003102.1 + 29043 0.66 0.995584
Target:  5'- uAUGAGACCGACGAcgacgugcacuacUCUagaGCGUcaGAUCa -3'
miRNA:   3'- -UACUUUGGCUGCU-------------AGGaa-UGCG--CUAG- -5'
11666 5' -49.3 NC_003102.1 + 4948 0.67 0.994912
Target:  5'- gAUGAcGCUGAUGAUUCggGCGUGGc- -3'
miRNA:   3'- -UACUuUGGCUGCUAGGaaUGCGCUag -5'
11666 5' -49.3 NC_003102.1 + 29412 0.67 0.994912
Target:  5'- -cGAcGCCGACGAgugugGCGgCGAUCa -3'
miRNA:   3'- uaCUuUGGCUGCUaggaaUGC-GCUAG- -5'
11666 5' -49.3 NC_003102.1 + 44609 0.67 0.994912
Target:  5'- aAUGucuGACCagauGGCGAUCUgagaacagACGCGAUCc -3'
miRNA:   3'- -UACu--UUGG----CUGCUAGGaa------UGCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 27510 0.67 0.99312
Target:  5'- uUGGAcaugGCCGAuCGGUUgg-ACGCGAUCg -3'
miRNA:   3'- uACUU----UGGCU-GCUAGgaaUGCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 89130 0.67 0.992053
Target:  5'- -aGAAAUCGAcCGAUCCgaaaacaACGCGGcUCu -3'
miRNA:   3'- uaCUUUGGCU-GCUAGGaa-----UGCGCU-AG- -5'
11666 5' -49.3 NC_003102.1 + 114301 0.67 0.990857
Target:  5'- gAUGGGACaCGcgcuagucaGAUUUUUGCGCGAUCa -3'
miRNA:   3'- -UACUUUG-GCug-------CUAGGAAUGCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 72137 0.67 0.990857
Target:  5'- gGUGuuuCUGGCGAUCgCgucCGCGAUCa -3'
miRNA:   3'- -UACuuuGGCUGCUAG-GaauGCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 134401 0.67 0.990857
Target:  5'- -aGAAuuuggcgccGCCGGCGGaaaauaUUUGCGCGAUCg -3'
miRNA:   3'- uaCUU---------UGGCUGCUag----GAAUGCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 33609 0.68 0.986409
Target:  5'- -aGAAACgcaCGACGAUCgUUGCaugagucgagGCGAUCu -3'
miRNA:   3'- uaCUUUG---GCUGCUAGgAAUG----------CGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 63604 0.69 0.980687
Target:  5'- cGUGGAACCGAUGgAUUUggaagaauugaacaGCGCGAUCg -3'
miRNA:   3'- -UACUUUGGCUGC-UAGGaa------------UGCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 88743 0.69 0.980462
Target:  5'- -aGAGAaCGugGAcgCgUUGCGCGAUCu -3'
miRNA:   3'- uaCUUUgGCugCUa-GgAAUGCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 18305 0.69 0.978102
Target:  5'- -cGAcGCCGACGAaCCUgccgccguCGCGAUg -3'
miRNA:   3'- uaCUuUGGCUGCUaGGAau------GCGCUAg -5'
11666 5' -49.3 NC_003102.1 + 133516 0.7 0.966536
Target:  5'- gAUGAAGCCGAaucgcUGAUCCgcguCGCcgGAUCg -3'
miRNA:   3'- -UACUUUGGCU-----GCUAGGaau-GCG--CUAG- -5'
11666 5' -49.3 NC_003102.1 + 95422 0.7 0.963074
Target:  5'- cAUGAGACCGGCauuGUCCgugGCGCaGAUg -3'
miRNA:   3'- -UACUUUGGCUGc--UAGGaa-UGCG-CUAg -5'
11666 5' -49.3 NC_003102.1 + 15705 0.72 0.92048
Target:  5'- -cGAcGCCGACGAgUC--GCGCGAUCg -3'
miRNA:   3'- uaCUuUGGCUGCUaGGaaUGCGCUAG- -5'
11666 5' -49.3 NC_003102.1 + 79279 0.78 0.660093
Target:  5'- gAUGGAACCGuACGAugugUCCgugacggACGCGAUCg -3'
miRNA:   3'- -UACUUUGGC-UGCU----AGGaa-----UGCGCUAG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.