Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11670 | 3' | -59.4 | NC_003102.1 | + | 72340 | 1.11 | 0.001023 |
Target: 5'- aAGCGGCCCGACAUCUCAGCGUCGCGCu -3' miRNA: 3'- -UCGCCGGGCUGUAGAGUCGCAGCGCG- -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 94801 | 0.72 | 0.397806 |
Target: 5'- uAGCGGCCCucucggccGACGUUUCGGUcgcgggaacgguucGUCGUGUa -3' miRNA: 3'- -UCGCCGGG--------CUGUAGAGUCG--------------CAGCGCG- -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 15869 | 0.69 | 0.556338 |
Target: 5'- uGGCGaucGCgCGAC-UCgUCGGCGUCGCGa -3' miRNA: 3'- -UCGC---CGgGCUGuAG-AGUCGCAGCGCg -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 62009 | 0.69 | 0.575092 |
Target: 5'- cGGCGGCUcggguguuucaauCGGCGUUUCGGCccGUCGCu- -3' miRNA: 3'- -UCGCCGG-------------GCUGUAGAGUCG--CAGCGcg -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 64876 | 0.69 | 0.595983 |
Target: 5'- uGCGGCCCaucagaaauGGCAUgUCGaacGCGUCGcCGUu -3' miRNA: 3'- uCGCCGGG---------CUGUAgAGU---CGCAGC-GCG- -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 72456 | 0.67 | 0.676028 |
Target: 5'- cAGCGacguGCCCGACAcggugUUUCAGCcGUCGUuCg -3' miRNA: 3'- -UCGC----CGGGCUGU-----AGAGUCG-CAGCGcG- -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 114538 | 0.67 | 0.70569 |
Target: 5'- cGCGucucuaaCCGAUAUCUUGuaGUCGCGCg -3' miRNA: 3'- uCGCcg-----GGCUGUAGAGUcgCAGCGCG- -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 95973 | 0.67 | 0.725181 |
Target: 5'- cGCGGUCUGAUcaAUC-CGGCGUUGUa- -3' miRNA: 3'- uCGCCGGGCUG--UAGaGUCGCAGCGcg -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 133773 | 0.66 | 0.734816 |
Target: 5'- gAGCGuCUgagagacgagCGGCGUCUCAGCcGUCGCa- -3' miRNA: 3'- -UCGCcGG----------GCUGUAGAGUCG-CAGCGcg -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 17379 | 0.66 | 0.744365 |
Target: 5'- uGCcGCCCGuGCuGUUUagcGCGUCGCGCa -3' miRNA: 3'- uCGcCGGGC-UG-UAGAgu-CGCAGCGCG- -5' |
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11670 | 3' | -59.4 | NC_003102.1 | + | 95538 | 0.66 | 0.772411 |
Target: 5'- ---cGCCCGA-AUCgaugccCAGCGUCGCGUu -3' miRNA: 3'- ucgcCGGGCUgUAGa-----GUCGCAGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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