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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 11677 | 3' | -51.7 | NC_003102.1 | + | 74347 | 0.66 | 0.991537 |
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Target: 5'- aCGC-AUCCGAGCGUCGAugcgaaaugauaaAUCcgUGcGUGGa -3' miRNA: 3'- -GCGcUGGGUUUGCAGCU-------------UAG--AC-CACC- -5' |
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| 11677 | 3' | -51.7 | NC_003102.1 | + | 108169 | 0.66 | 0.992093 |
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Target: 5'- cCGCGACCagacgcgGAAUGUUGAuuaaggacacgaacaAgcugCUGGUGGu -3' miRNA: 3'- -GCGCUGGg------UUUGCAGCU---------------Ua---GACCACC- -5' |
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| 11677 | 3' | -51.7 | NC_003102.1 | + | 80129 | 0.66 | 0.989134 |
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Target: 5'- aCGC-AUCgAAACGUgucUGAAUCgGGUGGg -3' miRNA: 3'- -GCGcUGGgUUUGCA---GCUUAGaCCACC- -5' |
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| 11677 | 3' | -51.7 | NC_003102.1 | + | 137019 | 0.67 | 0.984297 |
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Target: 5'- gGC-AUCCGAuCGUUGGGgcgccgCUGGUGGg -3' miRNA: 3'- gCGcUGGGUUuGCAGCUUa-----GACCACC- -5' |
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| 11677 | 3' | -51.7 | NC_003102.1 | + | 89510 | 0.7 | 0.9054 |
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Target: 5'- aCGUGGCUgAcuuuuCGUaGAAUCUGGUGGa -3' miRNA: 3'- -GCGCUGGgUuu---GCAgCUUAGACCACC- -5' |
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| 11677 | 3' | -51.7 | NC_003102.1 | + | 53227 | 0.72 | 0.839219 |
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Target: 5'- aGCGGCgCCAAaggaaGCGUCGA-UCUGGUc- -3' miRNA: 3'- gCGCUG-GGUU-----UGCAGCUuAGACCAcc -5' |
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| 11677 | 3' | -51.7 | NC_003102.1 | + | 53218 | 0.76 | 0.656081 |
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Target: 5'- gGCGcaguCUCGAagcaacAUGUCGAGUCUGGUGGc -3' miRNA: 3'- gCGCu---GGGUU------UGCAGCUUAGACCACC- -5' |
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| 11677 | 3' | -51.7 | NC_003102.1 | + | 103237 | 1.11 | 0.006156 |
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Target: 5'- aCGCGACCCAAACGUCGAAUCUGGUGGc -3' miRNA: 3'- -GCGCUGGGUUUGCAGCUUAGACCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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