Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11681 | 5' | -54.6 | NC_003102.1 | + | 132009 | 1.08 | 0.003918 |
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Target: 5'- uGUAUGCCGCGUCGCCUGUCGAUUCCAu -3' miRNA: 3'- -CAUACGGCGCAGCGGACAGCUAAGGU- -5' |
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| 11681 | 5' | -54.6 | NC_003102.1 | + | 134133 | 0.71 | 0.707604 |
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Target: 5'- -gAUGCacCGCaGUCGCUUGUCGAUguugUCCGc -3' miRNA: 3'- caUACG--GCG-CAGCGGACAGCUA----AGGU- -5' |
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| 11681 | 5' | -54.6 | NC_003102.1 | + | 134025 | 0.69 | 0.81342 |
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Target: 5'- cUcgGCCGCGUCGUcgCUGUCcAUcacUCCAa -3' miRNA: 3'- cAuaCGGCGCAGCG--GACAGcUA---AGGU- -5' |
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| 11681 | 5' | -54.6 | NC_003102.1 | + | 87604 | 0.68 | 0.847439 |
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Target: 5'- -gGUGCCaccgcCGUCGCCgacGUCGAUcgaucuUCCAu -3' miRNA: 3'- caUACGGc----GCAGCGGa--CAGCUA------AGGU- -5' |
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| 11681 | 5' | -54.6 | NC_003102.1 | + | 48862 | 0.67 | 0.885375 |
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Target: 5'- ---cGCCGCGUCGCUUGaaugCGA--CCu -3' miRNA: 3'- cauaCGGCGCAGCGGACa---GCUaaGGu -5' |
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| 11681 | 5' | -54.6 | NC_003102.1 | + | 133752 | 0.66 | 0.933905 |
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Target: 5'- ---cGCCGUaguGUCGCCgcaGUCGAgcgUCUg -3' miRNA: 3'- cauaCGGCG---CAGCGGa--CAGCUa--AGGu -5' |
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| 11681 | 5' | -54.6 | NC_003102.1 | + | 91337 | 0.66 | 0.938865 |
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Target: 5'- ---gGCCGCGUC-CgaGUCGAaUCUAc -3' miRNA: 3'- cauaCGGCGCAGcGgaCAGCUaAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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