miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
11684 5' -51.7 NC_003102.1 + 135630 0.66 0.983402
Target:  5'- -aCGAAuuGGAAGaguUGGAAGAaCGCGaaGCCGa -3'
miRNA:   3'- uaGCUU--CCUUC---ACCUUCU-GUGC--UGGC- -5'
11684 5' -51.7 NC_003102.1 + 118028 0.67 0.97125
Target:  5'- uAUCGGGuguuguGGaAAGUGGAcaagucGGACACGAUCa -3'
miRNA:   3'- -UAGCUU------CC-UUCACCU------UCUGUGCUGGc -5'
11684 5' -51.7 NC_003102.1 + 108504 0.66 0.983402
Target:  5'- -cCGGAGGuuuuuuGUcccauguauGcGGAGACACGACCGa -3'
miRNA:   3'- uaGCUUCCuu----CA---------C-CUUCUGUGCUGGC- -5'
11684 5' -51.7 NC_003102.1 + 102041 0.66 0.983402
Target:  5'- aAUCGAAGcGGcggcGGUGGcGGugGCGGCgGu -3'
miRNA:   3'- -UAGCUUC-CU----UCACCuUCugUGCUGgC- -5'
11684 5' -51.7 NC_003102.1 + 36857 0.69 0.930149
Target:  5'- --aGAAGGGAGUuaugaacaguucGGAGGAUcUGACCGa -3'
miRNA:   3'- uagCUUCCUUCA------------CCUUCUGuGCUGGC- -5'
11684 5' -51.7 NC_003102.1 + 12943 0.69 0.94034
Target:  5'- cUCGAaaAGGAAGUGGA---CACGACg- -3'
miRNA:   3'- uAGCU--UCCUUCACCUucuGUGCUGgc -5'
11684 5' -51.7 NC_003102.1 + 3478 1.08 0.007686
Target:  5'- aAUCGAAGGAAGUGGAAGACACGACCGg -3'
miRNA:   3'- -UAGCUUCCUUCACCUUCUGUGCUGGC- -5'
11684 5' -51.7 NC_003102.1 + 1622 0.75 0.674442
Target:  5'- gAUCGGAGGAGGcGG-AGGCAUGAUUGa -3'
miRNA:   3'- -UAGCUUCCUUCaCCuUCUGUGCUGGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.