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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11684 | 5' | -51.7 | NC_003102.1 | + | 135630 | 0.66 | 0.983402 |
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Target: 5'- -aCGAAuuGGAAGaguUGGAAGAaCGCGaaGCCGa -3' miRNA: 3'- uaGCUU--CCUUC---ACCUUCU-GUGC--UGGC- -5' |
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| 11684 | 5' | -51.7 | NC_003102.1 | + | 118028 | 0.67 | 0.97125 |
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Target: 5'- uAUCGGGuguuguGGaAAGUGGAcaagucGGACACGAUCa -3' miRNA: 3'- -UAGCUU------CC-UUCACCU------UCUGUGCUGGc -5' |
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| 11684 | 5' | -51.7 | NC_003102.1 | + | 108504 | 0.66 | 0.983402 |
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Target: 5'- -cCGGAGGuuuuuuGUcccauguauGcGGAGACACGACCGa -3' miRNA: 3'- uaGCUUCCuu----CA---------C-CUUCUGUGCUGGC- -5' |
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| 11684 | 5' | -51.7 | NC_003102.1 | + | 102041 | 0.66 | 0.983402 |
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Target: 5'- aAUCGAAGcGGcggcGGUGGcGGugGCGGCgGu -3' miRNA: 3'- -UAGCUUC-CU----UCACCuUCugUGCUGgC- -5' |
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| 11684 | 5' | -51.7 | NC_003102.1 | + | 36857 | 0.69 | 0.930149 |
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Target: 5'- --aGAAGGGAGUuaugaacaguucGGAGGAUcUGACCGa -3' miRNA: 3'- uagCUUCCUUCA------------CCUUCUGuGCUGGC- -5' |
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| 11684 | 5' | -51.7 | NC_003102.1 | + | 12943 | 0.69 | 0.94034 |
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Target: 5'- cUCGAaaAGGAAGUGGA---CACGACg- -3' miRNA: 3'- uAGCU--UCCUUCACCUucuGUGCUGgc -5' |
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| 11684 | 5' | -51.7 | NC_003102.1 | + | 3478 | 1.08 | 0.007686 |
|
Target: 5'- aAUCGAAGGAAGUGGAAGACACGACCGg -3' miRNA: 3'- -UAGCUUCCUUCACCUUCUGUGCUGGC- -5' |
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| 11684 | 5' | -51.7 | NC_003102.1 | + | 1622 | 0.75 | 0.674442 |
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Target: 5'- gAUCGGAGGAGGcGG-AGGCAUGAUUGa -3' miRNA: 3'- -UAGCUUCCUUCaCCuUCUGUGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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