Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11685 | 3' | -50.2 | NC_003102.1 | + | 101676 | 0.67 | 0.987717 |
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Target: 5'- -cGAUCGACCuGGUGAUGAaCGAUg- -3' miRNA: 3'- uuCUGGCUGGcCUACUACUaGUUAgc -5' |
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| 11685 | 3' | -50.2 | NC_003102.1 | + | 21515 | 0.67 | 0.984242 |
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Target: 5'- --cGCCGGCCGGAucgUGuaauUGAUCAAUa- -3' miRNA: 3'- uucUGGCUGGCCU---ACu---ACUAGUUAgc -5' |
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| 11685 | 3' | -50.2 | NC_003102.1 | + | 74386 | 0.68 | 0.980962 |
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Target: 5'- uGGAUCGACCGGAcGGUuucauacuuagcggcGAUCAGUUc -3' miRNA: 3'- uUCUGGCUGGCCUaCUA---------------CUAGUUAGc -5' |
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| 11685 | 3' | -50.2 | NC_003102.1 | + | 27517 | 0.69 | 0.954999 |
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Target: 5'- -uGGCCGAUCGGuugGAcgcGAUCGAUCa -3' miRNA: 3'- uuCUGGCUGGCCua-CUa--CUAGUUAGc -5' |
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| 11685 | 3' | -50.2 | NC_003102.1 | + | 4308 | 0.74 | 0.795312 |
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Target: 5'- aGAGAuuauauCCGAuCCGGAUG-UGAUCGAUCa -3' miRNA: 3'- -UUCU------GGCU-GGCCUACuACUAGUUAGc -5' |
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| 11685 | 3' | -50.2 | NC_003102.1 | + | 15022 | 0.76 | 0.71531 |
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Target: 5'- -uGACCGACCuaAUGAcGAUCAAUCGg -3' miRNA: 3'- uuCUGGCUGGccUACUaCUAGUUAGC- -5' |
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| 11685 | 3' | -50.2 | NC_003102.1 | + | 14945 | 0.79 | 0.534882 |
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Target: 5'- -cGACCGAUCGaAUGAUGAUCAAUCn -3' miRNA: 3'- uuCUGGCUGGCcUACUACUAGUUAGc -5' |
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| 11685 | 3' | -50.2 | NC_003102.1 | + | 14969 | 1.09 | 0.009619 |
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Target: 5'- aGAGACCGACCGGAUGAUGAUCAAUCGg -3' miRNA: 3'- -UUCUGGCUGGCCUACUACUAGUUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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