Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11685 | 5' | -49.6 | NC_003102.1 | + | 127404 | 0.68 | 0.986994 |
Target: 5'- -aCGAcgGcgGCCGAgaaUGggUGAUCGACg -3' miRNA: 3'- uaGCUuaC--UGGCUa--GCuuACUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 96506 | 0.66 | 0.997019 |
Target: 5'- cUCGAuucuaaccUGGCCGAcaaUCuGAUGAUCGGCg -3' miRNA: 3'- uAGCUu-------ACUGGCU---AGcUUACUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 90585 | 0.7 | 0.957107 |
Target: 5'- cUUGGAUGcCCGaAUCGAacgugGUGAUCGACu -3' miRNA: 3'- uAGCUUACuGGC-UAGCU-----UACUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 74792 | 0.66 | 0.996476 |
Target: 5'- gAUCGGgcacGUGACCacuUCGGAUG-CCGAa -3' miRNA: 3'- -UAGCU----UACUGGcu-AGCUUACuGGCUg -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 67312 | 0.71 | 0.939298 |
Target: 5'- aAUCGAAccUGGCCGAcuuuUCGAuaagcGAUCGACa -3' miRNA: 3'- -UAGCUU--ACUGGCU----AGCUua---CUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 63262 | 0.7 | 0.96451 |
Target: 5'- cUCGAGuuUGACaCGAUCGAucugGACCGuGCc -3' miRNA: 3'- uAGCUU--ACUG-GCUAGCUua--CUGGC-UG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 42486 | 0.71 | 0.939298 |
Target: 5'- -cCGggUGugCccguGAUCGAGUgGGCCGAUa -3' miRNA: 3'- uaGCuuACugG----CUAGCUUA-CUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 36838 | 0.76 | 0.730443 |
Target: 5'- uUCGGAggaucUGACCGAaUGGAUGGCCGAg -3' miRNA: 3'- uAGCUU-----ACUGGCUaGCUUACUGGCUg -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 27500 | 0.7 | 0.957107 |
Target: 5'- cUCGAccaacuuggacAUGGCCGAUCGGuugGACgCGAUc -3' miRNA: 3'- uAGCU-----------UACUGGCUAGCUua-CUG-GCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 24191 | 0.68 | 0.98336 |
Target: 5'- uAUCGAuauguAUGAagCGAUCGAuccaaaGACCGGCg -3' miRNA: 3'- -UAGCU-----UACUg-GCUAGCUua----CUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 24126 | 0.66 | 0.996476 |
Target: 5'- aGUCgGAAUGAUCGAUgGucAUGGCCG-Cu -3' miRNA: 3'- -UAG-CUUACUGGCUAgCu-UACUGGCuG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 21296 | 0.67 | 0.993333 |
Target: 5'- cAUCGccgccGACCGAUCGAgAUGguaacauGCCGGCu -3' miRNA: 3'- -UAGCuua--CUGGCUAGCU-UAC-------UGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 15003 | 1.08 | 0.013664 |
Target: 5'- gAUCGAAUGACCGAUCGAAUGACCGACc -3' miRNA: 3'- -UAGCUUACUGGCUAGCUUACUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 14941 | 0.73 | 0.850402 |
Target: 5'- uUCGc--GACCGAUCGAAUgaugaucaaucagaGACCGACc -3' miRNA: 3'- uAGCuuaCUGGCUAGCUUA--------------CUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 12681 | 0.7 | 0.948714 |
Target: 5'- cUCG-GUGACCGuUCGAAuuuUGuCCGACg -3' miRNA: 3'- uAGCuUACUGGCuAGCUU---ACuGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 9559 | 0.68 | 0.988565 |
Target: 5'- uUCGAGUcGGgCGAUCGuugagcgGACCGAUc -3' miRNA: 3'- uAGCUUA-CUgGCUAGCuua----CUGGCUG- -5' |
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11685 | 5' | -49.6 | NC_003102.1 | + | 5579 | 0.71 | 0.944136 |
Target: 5'- uUCGAuUGua-GGUCGAgAUGACCGACg -3' miRNA: 3'- uAGCUuACuggCUAGCU-UACUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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