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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 11688 | 3' | -60.7 | NC_003102.1 | + | 34215 | 0.66 | 0.666349 |
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Target: 5'- aAUGG-GUGCCCGUCUUGAugcguuuucCCUGUa-- -3' miRNA: 3'- -UGCCgCACGGGCAGAGCU---------GGACGcug -5' |
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| 11688 | 3' | -60.7 | NC_003102.1 | + | 93580 | 0.66 | 0.686098 |
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Target: 5'- cGCGGCGcuCUCGcCUCGACC-GuCGACg -3' miRNA: 3'- -UGCCGCacGGGCaGAGCUGGaC-GCUG- -5' |
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| 11688 | 3' | -60.7 | NC_003102.1 | + | 100915 | 0.67 | 0.642516 |
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Target: 5'- gACGGCGgcucggugaucugGCCCagGUCUCGuuCUGCGu- -3' miRNA: 3'- -UGCCGCa------------CGGG--CAGAGCugGACGCug -5' |
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| 11688 | 3' | -60.7 | NC_003102.1 | + | 64144 | 0.67 | 0.656431 |
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Target: 5'- cUGGuCGUGCCCgGUCUCagcgGAUUcggaUGCGACa -3' miRNA: 3'- uGCC-GCACGGG-CAGAG----CUGG----ACGCUG- -5' |
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| 11688 | 3' | -60.7 | NC_003102.1 | + | 81368 | 0.67 | 0.656431 |
|
Target: 5'- -gGGCGUGCUcaacgacgcguCGUCUgCGACg-GCGACc -3' miRNA: 3'- ugCCGCACGG-----------GCAGA-GCUGgaCGCUG- -5' |
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| 11688 | 3' | -60.7 | NC_003102.1 | + | 48991 | 0.68 | 0.566239 |
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Target: 5'- gGCGGCGaaacGCCCGUUcaccaucgauccgUCauGACCaGCGACg -3' miRNA: 3'- -UGCCGCa---CGGGCAG-------------AG--CUGGaCGCUG- -5' |
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| 11688 | 3' | -60.7 | NC_003102.1 | + | 17306 | 0.73 | 0.29039 |
|
Target: 5'- uGCGGCGUGCCCGcCaUCGACauacccGcCGACg -3' miRNA: 3'- -UGCCGCACGGGCaG-AGCUGga----C-GCUG- -5' |
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| 11688 | 3' | -60.7 | NC_003102.1 | + | 23136 | 1.08 | 0.001202 |
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Target: 5'- uACGGCGUGCCCGUCUCGACCUGCGACu -3' miRNA: 3'- -UGCCGCACGGGCAGAGCUGGACGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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