Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11689 | 3' | -50.3 | NC_003102.1 | + | 3544 | 0.66 | 0.993189 |
Target: 5'- -uCAGGAuacAGACGCGUCGUguUACauuccgauauuguGGCc -3' miRNA: 3'- uuGUCCU---UUUGUGCAGCGguAUG-------------CCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 15405 | 0.66 | 0.994037 |
Target: 5'- cGGCAGGAucguugucaACGUCGCUGgucUugGGUg -3' miRNA: 3'- -UUGUCCUuuug-----UGCAGCGGU---AugCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 21326 | 0.69 | 0.963966 |
Target: 5'- uGCcGGcu-GCGCGUaCGCC-UGCGGCg -3' miRNA: 3'- uUGuCCuuuUGUGCA-GCGGuAUGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 21544 | 0.7 | 0.927942 |
Target: 5'- uGugAGGAAAGCgACGgCGCCAU-CGGg -3' miRNA: 3'- -UugUCCUUUUG-UGCaGCGGUAuGCCg -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 23980 | 0.72 | 0.867586 |
Target: 5'- uACAuGAugauGCGCGUCGCCAU-CGGUa -3' miRNA: 3'- uUGUcCUuu--UGUGCAGCGGUAuGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 29463 | 1.11 | 0.006358 |
Target: 5'- gAACAGGAAAACACGUCGCCAUACGGCa -3' miRNA: 3'- -UUGUCCUUUUGUGCAGCGGUAUGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 33137 | 0.67 | 0.984802 |
Target: 5'- uGACGGGAAcguuucGGCGCGUUGCUgauucuuGUGCuGCu -3' miRNA: 3'- -UUGUCCUU------UUGUGCAGCGG-------UAUGcCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 41471 | 0.69 | 0.956081 |
Target: 5'- cGGCGGcGAGGACaagauucGCGUCGUCGacUACGGUu -3' miRNA: 3'- -UUGUC-CUUUUG-------UGCAGCGGU--AUGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 61979 | 0.66 | 0.994214 |
Target: 5'- -cCAGGGAAugucCAUGaUCGUCGgucgagGCGGCg -3' miRNA: 3'- uuGUCCUUUu---GUGC-AGCGGUa-----UGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 62994 | 0.67 | 0.988341 |
Target: 5'- cGAgAGGAuuucGCAUGUCGCgCGUggccaaaacgACGGCc -3' miRNA: 3'- -UUgUCCUuu--UGUGCAGCG-GUA----------UGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 70554 | 0.67 | 0.986746 |
Target: 5'- -uCAGcuuGGACACGUCGUCG-ACGGUc -3' miRNA: 3'- uuGUCcu-UUUGUGCAGCGGUaUGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 71808 | 0.68 | 0.978881 |
Target: 5'- cAACGGuuucuuaccuccGCGCGUCGCCAgauCGGCc -3' miRNA: 3'- -UUGUCcuuu--------UGUGCAGCGGUau-GCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 78008 | 0.66 | 0.993672 |
Target: 5'- cAACAGGAAAAUuuuaggagcgACGUCGUCGaaaacgugaccagcAUGGCg -3' miRNA: 3'- -UUGUCCUUUUG----------UGCAGCGGUa-------------UGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 84597 | 0.66 | 0.994214 |
Target: 5'- cAACAuGGAGGAaGCGcUCGCUuccauggACGGCg -3' miRNA: 3'- -UUGU-CCUUUUgUGC-AGCGGua-----UGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 88111 | 0.73 | 0.825685 |
Target: 5'- cGGCAGGAG---GCGUCGCaguucggGCGGCa -3' miRNA: 3'- -UUGUCCUUuugUGCAGCGgua----UGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 103328 | 0.66 | 0.995034 |
Target: 5'- aAACcGGAAAgacGCGCGUCGUCAaGCGu- -3' miRNA: 3'- -UUGuCCUUU---UGUGCAGCGGUaUGCcg -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 106838 | 0.66 | 0.991082 |
Target: 5'- uGGCGGGuuAAACGCGccgcuaccguuUCGUCAaACGGUa -3' miRNA: 3'- -UUGUCCu-UUUGUGC-----------AGCGGUaUGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 117632 | 0.7 | 0.92227 |
Target: 5'- uACAcGGA--ACACGUCGCCA---GGCg -3' miRNA: 3'- uUGU-CCUuuUGUGCAGCGGUaugCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 120459 | 0.66 | 0.994214 |
Target: 5'- -uUAGGAuccgAAGCGCGcuuucagcgcUCGUguUACGGCg -3' miRNA: 3'- uuGUCCU----UUUGUGC----------AGCGguAUGCCG- -5' |
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11689 | 3' | -50.3 | NC_003102.1 | + | 120609 | 0.74 | 0.777968 |
Target: 5'- ---cGGGAAGCACGuUCGCCAcaauguccgcgucUGCGGUg -3' miRNA: 3'- uuguCCUUUUGUGC-AGCGGU-------------AUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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