Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1169 | 5' | -52.1 | NC_001132.2 | + | 46392 | 0.66 | 0.988347 |
Target: 5'- uACGUCGAgguGCGugGUUgUCUCUaaaaagggcaaCACCGa -3' miRNA: 3'- -UGUAGCU---UGCugCAGaAGAGG-----------GUGGU- -5' |
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1169 | 5' | -52.1 | NC_001132.2 | + | 152007 | 0.66 | 0.983064 |
Target: 5'- cACGUCGccagaugGACGGCGUg-UCUCCUACg- -3' miRNA: 3'- -UGUAGC-------UUGCUGCAgaAGAGGGUGgu -5' |
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1169 | 5' | -52.1 | NC_001132.2 | + | 57516 | 0.68 | 0.961663 |
Target: 5'- gACAUC--GCGACGUCgucCUCCUguGCCGc -3' miRNA: 3'- -UGUAGcuUGCUGCAGaa-GAGGG--UGGU- -5' |
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1169 | 5' | -52.1 | NC_001132.2 | + | 82783 | 0.69 | 0.945651 |
Target: 5'- gGCGuuUCGAACGuCGUCaUCUCugaaaCCACCGc -3' miRNA: 3'- -UGU--AGCUUGCuGCAGaAGAG-----GGUGGU- -5' |
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1169 | 5' | -52.1 | NC_001132.2 | + | 9050 | 0.71 | 0.88156 |
Target: 5'- aACGagCGAACGACGUCUccugUCacuUCgCCACCAu -3' miRNA: 3'- -UGUa-GCUUGCUGCAGA----AG---AG-GGUGGU- -5' |
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1169 | 5' | -52.1 | NC_001132.2 | + | 155703 | 0.71 | 0.88156 |
Target: 5'- cCAUCGcguUGACGUCggCUCCCGCa- -3' miRNA: 3'- uGUAGCuu-GCUGCAGaaGAGGGUGgu -5' |
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1169 | 5' | -52.1 | NC_001132.2 | + | 153916 | 0.71 | 0.874296 |
Target: 5'- cGCcgUGAACGACGUCUcC-CCCGCg- -3' miRNA: 3'- -UGuaGCUUGCUGCAGAaGaGGGUGgu -5' |
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1169 | 5' | -52.1 | NC_001132.2 | + | 139825 | 1 | 0.030135 |
Target: 5'- uACAUCG-ACGACGUCUUCUCCCACCAa -3' miRNA: 3'- -UGUAGCuUGCUGCAGAAGAGGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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