Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11690 | 3' | -59.3 | NC_003102.1 | + | 126160 | 0.66 | 0.793005 |
Target: 5'- -aUGGCGGCgCGACCG-UGAucaaguuuauaACGGGUc -3' miRNA: 3'- agGCUGCCGaGCUGGCcACU-----------UGCCCA- -5' |
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11690 | 3' | -59.3 | NC_003102.1 | + | 1726 | 0.66 | 0.784131 |
Target: 5'- -gUGAUGGUUCGAUCGGUGGcuccgGCaGGa -3' miRNA: 3'- agGCUGCCGAGCUGGCCACU-----UGcCCa -5' |
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11690 | 3' | -59.3 | NC_003102.1 | + | 41146 | 0.66 | 0.766006 |
Target: 5'- aCgGAUGGCgCGAUCGGUGGuguACGGu- -3' miRNA: 3'- aGgCUGCCGaGCUGGCCACU---UGCCca -5' |
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11690 | 3' | -59.3 | NC_003102.1 | + | 48819 | 0.68 | 0.640253 |
Target: 5'- -aUGACGGaUCGA-UGGUGAACGGGc -3' miRNA: 3'- agGCUGCCgAGCUgGCCACUUGCCCa -5' |
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11690 | 3' | -59.3 | NC_003102.1 | + | 42219 | 0.74 | 0.327806 |
Target: 5'- gCCGACGGCU-GuuCGGUGAGCGcGGa -3' miRNA: 3'- aGGCUGCCGAgCugGCCACUUGC-CCa -5' |
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11690 | 3' | -59.3 | NC_003102.1 | + | 41019 | 1.07 | 0.002219 |
Target: 5'- gUCCGACGGCUCGACCGGUGAACGGGUu -3' miRNA: 3'- -AGGCUGCCGAGCUGGCCACUUGCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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