Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11693 | 5' | -52 | NC_003102.1 | + | 129190 | 0.68 | 0.949322 |
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Target: 5'- aCGCaggacgaguAGCGGCGgcCGGC--GCCGACg -3' miRNA: 3'- -GCGaau------UUGCCGCa-GCCGaaUGGCUG- -5' |
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| 11693 | 5' | -52 | NC_003102.1 | + | 23695 | 0.67 | 0.964723 |
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Target: 5'- gGCgUAGGCGGCGcguccaaCGGUcucgUGCCGAUg -3' miRNA: 3'- gCGaAUUUGCCGCa------GCCGa---AUGGCUG- -5' |
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| 11693 | 5' | -52 | NC_003102.1 | + | 95197 | 0.67 | 0.967991 |
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Target: 5'- aGCUUGgugcacgccGACGacGCGUCuuuGGCgucUACCGACg -3' miRNA: 3'- gCGAAU---------UUGC--CGCAG---CCGa--AUGGCUG- -5' |
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| 11693 | 5' | -52 | NC_003102.1 | + | 95897 | 0.67 | 0.973873 |
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Target: 5'- aCGC--GAACGGCGUCuaCUcgGCCGAUu -3' miRNA: 3'- -GCGaaUUUGCCGCAGccGAa-UGGCUG- -5' |
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| 11693 | 5' | -52 | NC_003102.1 | + | 121269 | 0.66 | 0.985099 |
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Target: 5'- cCGC----ACGGCGUCGuc--GCCGACa -3' miRNA: 3'- -GCGaauuUGCCGCAGCcgaaUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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