Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11696 | 5' | -55.6 | NC_003102.1 | + | 134386 | 0.66 | 0.920849 |
Target: 5'- gCGGCGGCGGUCgcaagaauuuggCGCCGCcg--GCGGa -3' miRNA: 3'- -GCUGCUGUCGGa-----------GCGGCGucuaUGUC- -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 133870 | 0.66 | 0.893829 |
Target: 5'- gCGACGACGcggccgagcugcaucGCCUUGCCGUcGGUGa-- -3' miRNA: 3'- -GCUGCUGU---------------CGGAGCGGCGuCUAUguc -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 130895 | 0.68 | 0.804153 |
Target: 5'- uGGCGACGGCgUCGCUGUguguagcauuugGGAUAUu- -3' miRNA: 3'- gCUGCUGUCGgAGCGGCG------------UCUAUGuc -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 129177 | 0.72 | 0.616466 |
Target: 5'- -aGCGGCGGCCggCGCCGaCGGA-GCAGu -3' miRNA: 3'- gcUGCUGUCGGa-GCGGC-GUCUaUGUC- -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 129101 | 0.69 | 0.776692 |
Target: 5'- gCGGCGGCGGCCga--CGCAGGaccgGCAGu -3' miRNA: 3'- -GCUGCUGUCGGagcgGCGUCUa---UGUC- -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 90052 | 0.67 | 0.854609 |
Target: 5'- cCGAUGACAuccuGUCUgGCgGCGGcgGCGGa -3' miRNA: 3'- -GCUGCUGU----CGGAgCGgCGUCuaUGUC- -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 88040 | 0.67 | 0.876467 |
Target: 5'- gCGGCGAUgguagaaGGCgUCgaaGCCGCAGucauUACAGa -3' miRNA: 3'- -GCUGCUG-------UCGgAG---CGGCGUCu---AUGUC- -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 81414 | 1.09 | 0.002928 |
Target: 5'- uCGACGACAGCCUCGCCGCAGAUACAGa -3' miRNA: 3'- -GCUGCUGUCGGAGCGGCGUCUAUGUC- -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 77314 | 0.7 | 0.738291 |
Target: 5'- uGACGACuauugcgaAGCCgUCGUCGUAGAUAUc- -3' miRNA: 3'- gCUGCUG--------UCGG-AGCGGCGUCUAUGuc -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 35753 | 0.67 | 0.877189 |
Target: 5'- cCGAUGACAGuUCUCGCgGC-GAUAUc- -3' miRNA: 3'- -GCUGCUGUC-GGAGCGgCGuCUAUGuc -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 17733 | 0.67 | 0.862347 |
Target: 5'- gCGGCGGCGGCggCgGCgGCGGcgGCGGu -3' miRNA: 3'- -GCUGCUGUCGgaG-CGgCGUCuaUGUC- -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 17464 | 0.68 | 0.830199 |
Target: 5'- uCGAUGGCGGgCaCGCCGCAcgaucGGUACAu -3' miRNA: 3'- -GCUGCUGUCgGaGCGGCGU-----CUAUGUc -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 16415 | 0.66 | 0.897783 |
Target: 5'- aCGACGACGGgCgucugguugaUCGCCGC-GAUucugccGCAGu -3' miRNA: 3'- -GCUGCUGUCgG----------AGCGGCGuCUA------UGUC- -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 15811 | 0.7 | 0.708476 |
Target: 5'- -cACGAC-GUCUCGCCGC-GAUACGu -3' miRNA: 3'- gcUGCUGuCGGAGCGGCGuCUAUGUc -5' |
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11696 | 5' | -55.6 | NC_003102.1 | + | 13067 | 0.72 | 0.606193 |
Target: 5'- aGACGgaaACGGUCUCGCCGUAGAcauCAa -3' miRNA: 3'- gCUGC---UGUCGGAGCGGCGUCUau-GUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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