Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11697 | 5' | -53.7 | NC_003102.1 | + | 18504 | 0.66 | 0.962813 |
Target: 5'- cGAUGUUCGCGCCaAUC-UG-CCGgauguaacCGAa -3' miRNA: 3'- -CUGCAAGCGCGG-UAGuACaGGCa-------GCU- -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 70734 | 0.66 | 0.951427 |
Target: 5'- aACGaagCGCGCCAgc-UGaCCGUCGAc -3' miRNA: 3'- cUGCaa-GCGCGGUaguACaGGCAGCU- -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 34794 | 0.67 | 0.94266 |
Target: 5'- aACGUUUGCGCCu---UGaUCGUCGAc -3' miRNA: 3'- cUGCAAGCGCGGuaguACaGGCAGCU- -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 97176 | 0.68 | 0.916482 |
Target: 5'- uACGg--GCGCuCAUagaucuucgCAUGUCCGUCGAg -3' miRNA: 3'- cUGCaagCGCG-GUA---------GUACAGGCAGCU- -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 103082 | 0.68 | 0.904297 |
Target: 5'- cGACGUuugggUCGCGUCcgcgGUCAUGUCCa-CGAu -3' miRNA: 3'- -CUGCA-----AGCGCGG----UAGUACAGGcaGCU- -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 80084 | 0.69 | 0.862089 |
Target: 5'- uGACauggUCGaCGCCAUCGUG-CCGUCc- -3' miRNA: 3'- -CUGca--AGC-GCGGUAGUACaGGCAGcu -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 88907 | 0.7 | 0.845443 |
Target: 5'- gGACGgugUCGUguccgaaGCCGUCGgcgCCGUCGAa -3' miRNA: 3'- -CUGCa--AGCG-------CGGUAGUacaGGCAGCU- -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 78565 | 0.7 | 0.838037 |
Target: 5'- cGCGaUCGCacGCCGUCAuUGUCUGUCa- -3' miRNA: 3'- cUGCaAGCG--CGGUAGU-ACAGGCAGcu -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 114075 | 0.7 | 0.838037 |
Target: 5'- cGACGUgcUUGCGuCCAUUcgGUCCGUa-- -3' miRNA: 3'- -CUGCA--AGCGC-GGUAGuaCAGGCAgcu -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 113425 | 0.71 | 0.775571 |
Target: 5'- cGACaGUUCGaUGCCAUCAUuccuaugaaaaGUCCGUCu- -3' miRNA: 3'- -CUG-CAAGC-GCGGUAGUA-----------CAGGCAGcu -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 39210 | 0.75 | 0.55216 |
Target: 5'- cGACGUUUGCGUCGUCuGUGUucaccgaaUCGUCGAu -3' miRNA: 3'- -CUGCAAGCGCGGUAG-UACA--------GGCAGCU- -5' |
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11697 | 5' | -53.7 | NC_003102.1 | + | 85921 | 1.1 | 0.00434 |
Target: 5'- cGACGUUCGCGCCAUCAUGUCCGUCGAa -3' miRNA: 3'- -CUGCAAGCGCGGUAGUACAGGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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