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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 11698 | 3' | -53.1 | NC_003102.1 | + | 87971 | 0.66 | 0.964511 |
|
Target: 5'- cGCCUGAGCUuCGGCGUCUu------- -3' miRNA: 3'- -CGGGCUCGAcGCUGCAGAcauuacug -5' |
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| 11698 | 3' | -53.1 | NC_003102.1 | + | 37879 | 0.66 | 0.957291 |
|
Target: 5'- cUUCGGGCgGCGGCGgcggCggcgGUGAUGAUg -3' miRNA: 3'- cGGGCUCGaCGCUGCa---Ga---CAUUACUG- -5' |
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| 11698 | 3' | -53.1 | NC_003102.1 | + | 39549 | 0.67 | 0.935045 |
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Target: 5'- cUCCGA-CUGCGACGUCUcGU-AUGGa -3' miRNA: 3'- cGGGCUcGACGCUGCAGA-CAuUACUg -5' |
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| 11698 | 3' | -53.1 | NC_003102.1 | + | 790 | 0.67 | 0.949127 |
|
Target: 5'- cGCCUGAGCUGUGAaauuuucuCGUCU----UGAUc -3' miRNA: 3'- -CGGGCUCGACGCU--------GCAGAcauuACUG- -5' |
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| 11698 | 3' | -53.1 | NC_003102.1 | + | 49653 | 0.7 | 0.856485 |
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Target: 5'- cGCCCGAucaacGCgacGCGGCGUUUGUAAa--- -3' miRNA: 3'- -CGGGCU-----CGa--CGCUGCAGACAUUacug -5' |
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| 11698 | 3' | -53.1 | NC_003102.1 | + | 87845 | 1.12 | 0.003067 |
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Target: 5'- cGCCCGAGCUGCGACGUCUGUAAUGACu -3' miRNA: 3'- -CGGGCUCGACGCUGCAGACAUUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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